Human/Equine CD11b/Integrin alpha M Antibody

Catalog # Availability Size / Price Qty
MAB16991
MAB16991-SP
Human/Equine CD11b/Integrin alpha M Antibody in Data
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Human/Equine CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Human, Equine
Specificity
Detects human CD11b/Integrin alpha M in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 238446
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M
Phe17-Asn1105
Accession # NP_001139280
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD11b/Integrin  alpha M in Equine PBMCs by Flow Cytometry. View Larger

Detection of CD11b/Integrin alpha M in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human/Equine CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB16991, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry CD11b/Integrin  alpha M in Human PBMCs. View Larger

CD11b/Integrin alpha M in Human PBMCs. CD11b/Integrin aM was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human/Equine CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB16991) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD11b/Integrin alpha M in Human Brain. View Larger

CD11b/Integrin alpha M in Human Brain. CD11b/Integrin alpha M was detected in immersion fixed paraffin-embedded sections of human brain (cerebral cortex) using Mouse Anti-Human/Equine CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16991). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of microglia. Tissue was co-stained with using a Sheep Anti-Human/Mouse/Rat Neurogranin Antigen Affinity-Purified Polyclonal Anitbody (Catalog # AF7947) and an Alexa Fluor® 488-conjugated Donkey Anti-Sheep IgG Secondary Antibody (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin alpha M subunit (CD11b), associates with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimeric Integrin CD11b/CD18, also known as Mac-1 and CR-3. Upon activation, CD11b/CD18 is expressed on granulocytes, monocytes, a subset of NK cells and activated lymphocytes. Integrin CD11b/CD18 functions as a receptor for complement fragment iC3b, ICAM-1 (CD54), ICAM-2 (CD102) and fibrinogen to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1‑3).

References
  1. Springer, T.A. et al. (1978) Eur. J. Immunol. 8:539.
  2. Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
  3. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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