Human/Equine CD11b/Integrin alpha M Antibody

Catalog # Availability Size / Price Qty
MAB16991
MAB16991-SP
Detection of CD11b/Integrin alpha M in PBMC monocytes by Flow Cytometry.
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Product Details
Citations (3)
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Reviews (1)

Human/Equine CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Human, Equine
Specificity
Detects human CD11b/Integrin alpha M in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 238446
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD11b/Integrin alpha M
Phe17-Asn1105
Accession # NP_001139280
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
PBMC monocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of CD11b/Integrin alpha M in PBMC monocytes by Flow Cytometry. PBMC monocytes were stained with Mouse Anti-Human/Equine CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB16991, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin alpha M subunit (CD11b), associates with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimeric Integrin CD11b/CD18, also known as Mac-1 and CR-3. Upon activation, CD11b/CD18 is expressed on granulocytes, monocytes, a subset of NK cells and activated lymphocytes. Integrin CD11b/CD18 functions as a receptor for complement fragment iC3b, ICAM-1 (CD54), ICAM-2 (CD102) and fibrinogen to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles (1‑3).

References
  1. Springer, T.A. et al. (1978) Eur. J. Immunol. 8:539.
  2. Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
  3. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

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Citations for Human/Equine CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Advances in Visualizing Microglial Cells in Human Central Nervous System Tissue
    Authors: Uff CEG, Patel K, Yeung C, Yip PK
    Biomolecules
  2. Cell Adhesion Molecules are Upregulated and May Drive Inflammation in Chronic Rhinosinusitis with Nasal Polyposis
    Authors: Brennan J Blight, Amarbir S Gill, Jorgen S Sumsion, Chelsea E Pollard, Shaelene Ashby, Gretchen M Oakley et al.
    Journal of Asthma and Allergy
  3. The human tissue-biomaterial interface: a role for PPAR gamma -dependent glucocorticoid receptor activation in regulating the CD163+ M2 macrophage phenotype
    Authors: Samuel J. Bullers, Simon C. Baker, Eileen Ingham, Jennifer Southgate
    Tissue Engineering Part A

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Reviews for Human/Equine CD11b/Integrin alpha M Antibody

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Human/Equine CD11b/Integrin alpha M Antibody
By Hannah Kemelmakher on 05/09/2022
Application: IHC Sample Tested: Lymph node tissue,synovial tissue Species: Equine

Paraffin-fixed tissue was subjected to HEIR then incubated with primary antibody and anti-mouse binding protein conjugated to HRP prior to counterstaining and mounting.