Human Kallikrein 11 Antibody

Catalog # Availability Size / Price Qty
MAB15951
MAB15951-SP
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Human Kallikrein 11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Kallikrein 11 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 371604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Kallikrein 11
Glu51-Asn282
Accession # Q9UBX7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize Recombinant Human Kallikrein 11 (1 µg/mL, Catalog # 1595-SE) cleavage of the fluorogenic peptide substrate VLK-SBzl (0.1 mM). The Neutralization Dose (ND50) is typically 2.4 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kallikrein 11

As a member of human tissue kallikrein family, Kallikrein 11, also known as hippostasin, trypsin-like serine protease and PRSS20, is encoded by the KLK11 gene (1). Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences,respectively (2-5). Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain (residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue 1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas (6-8). Recombinant human (rh) KLK11, after being activated by thermolysin, is active against a thioester substrate. This activity can be inhibited by AEBSF (R&D Systems, Catalog # EI001), dichloroisocoumarin, and aprotinin. rhKLK11 produced by R&D Systems corresponds to isoform 1.

References
  1. Yousef, G.M. and E.P. Diamandis (2001) Endocrine Rev. 22:184.
  2. Yoshida, S. et al. (1998) Biochim. Biophys. Acta 1399:225. 
  3. Yousef, G.M. et al. (2000) Genomics 63:88.
  4. Mitsui, S. et al. (2000) Biochem. Biophys. Res. Commun. 272:205.
  5. Gan, L. et al. (2000) Gene 257:119.
  6. Diamandis, E.P. et al. (2002) Cancer Res. 62:295.
  7. Nakamura, T. et al. (2003) Urology 61:1042.
  8. Borgono, C.A. et al. (2003) Int. J. Cancer 106:605.
Entrez Gene IDs
11012 (Human)
Alternate Names
EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; Hippostasin; hK11; Kallikrein 11; kallikrein-related peptidase 11; KLK11; MGC33060; PRSS20kallikrein-11; Serine protease 20; TLSPhippostasin; Trypsin-like protease

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