Rat IgG2A Isotype Control

Recombinant Monoclonal Antibody
  
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  • Specificity
    Selected for use as rat IgG2A isotype control. In direct ELISAs, does not cross-react with recombinant human (rh) 6Ckine, recombinant mouse (rm) 6Ckine, rhACT II (rhMIP-1 beta ), rhBLC/BCA-1, rmBLC, rmC10, recombinant rat (rr) CINC-1, rrCINC-2 alpha, rrCINC-2 beta, rrCINC-3, rhCK beta 8-1, recombinant viral (rv) CMV UL146, rmCRG-2, rhENA-78, rhEotaxin, rmEotaxin, rhEotaxin 2, rhEotaxin-3, rmEotaxin 2, rhFractalkine, rmFractalkine, rrFractalkine, rhGCP-2, rmGCP-2, rhGRO alpha, rhGRO beta, rhGRO gamma, rhHCC-1, rhHCC-4, rhI-309, rhIL-8, recombinant porcine IL-8, rhIP-10, rhI-TAC, rmJE, rmKC, rhLeukotactin -1, rhLymphytactin, rmLymphotactin, rmMARC, rhMCP-1, rhMCP-2, rhMCP-3, rhMCP-4, rmMCP-5, rvMCV Type 2, rhMDC, rmMDC, rhMIG, rmMIG, rhMIP-1 alpha, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma, rhMIP-1δ, rmMIP-2, rhMIP-3 alpha, rrMIP-3 alpha, rhMIP-3 beta, rmMIP-3 alpha, rmMIP-3 beta, rvMIP-I, rvMIP-II, rvMIP-III, rhMPIF-1, rhNAP-2, rhParc, rhRANTES, rmRANTES, rhSDF-1 alpha, rmSDF-1 alpha, rhSDF-1 beta, rhTarc, rmTARC, rmTCA-3, rhTeck, or rmTeck.
  • Source
    Monoclonal Rat IgG2A Clone # 54447R
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    KLH
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for use in conjunction with R&D Systems antibodies. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGControl by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae‑1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006R, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2A Fc
  • Alternate Names:
    IgG2A

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 91  
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Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Rat IgG2A Allophycocyanin Isotype Control

Ctrl IC006A 7  
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Rat IgG2A Fluorescein-conjugated Antibody

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Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Rat IgG2A Alexa Fluor® 594-conjugated Antibody

Ctrl IC006T
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Rat IgG2A Alexa Fluor® 647-conjugated Antibody

Ctrl IC006R
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Rat IgG2A Alexa Fluor® 750-conjugated Antibody

Ctrl IC006S
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Rat IgG2A Biotinylated Isotype Control

Ctrl IC006B
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Rat IgG2A Alexa Fluor® 405-conjugated Antibody

Ctrl IC006V
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Rat IgG2A PerCP‑conjugated Control Antibody

Ctrl IC006C
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Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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