Human Lactate Dehydrogenase B/LDHB Alexa Fluor® 594-conjugated Antibody

Recombinant Monoclonal Antibody
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R&D Systems Antibodies
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Product Details

Human Lactate Dehydrogenase B/LDHB Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Detects human LDHB in direct ELISAs and Western blots.
Monoclonal Rabbit IgG Clone # 2057D
E. coli-derived recombinant human LDHB
Accession # P07195
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
HeLa human cervical epithelial carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lactate Dehydrogenase B/LDHB

LDHB is one of two subunits of Lactose Dehydrogenase which is formed from tetramer arrangements of the A and B subunits.  LDHB is part of the pyruvate fermentation to lactate pathway in which it catalyzes the conversion of L-lactate and NAD to pyruvate and NADH.  LDHA is widely expressed, with high expression in muscle and brain.  High expression in tumor cells correlates with low survival rates in various cancers including osteosarcoma, oral squamous cell carcinoma breast and lung cancers. Unlike Lactose Dehyrogenase A, LDHB mutations are not linked to any disease

Entrez Gene IDs
3945 (Human); 16832 (Mouse); 24534 (Rat)
Alternate Names
EC 1.1.1; EC; Epididymis Secretory Protein Li 281; HEL-S-281; Lactate Dehydrogenase B; LDH heart subunit; LDHB; LDH-B; LDHBD; LDH-H; L-lactate dehydrogenase B chain; Renal Carcinoma Antigen NY-REN-46; TRG-5

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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