Human Lactate Dehydrogenase B/LDHB Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB9205-100
MAB9205-SP
Detection of Human Lactate Dehydrogenase B/LDHB by Western Blot.
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human Lactate Dehydrogenase B/LDHB Antibody Summary

Species Reactivity
Human
Specificity
Detects human LDHB in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2057D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human LDHB
Met1-Val93
Accession # P07195
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below
Knockout Validated
Lactate Dehydrogenase B/LDHB is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in Lactate Dehydrogenase B/LDHB knockout HEK293Tcell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Lactate Dehydrogenase B/LDHB antibody by Western Blot. View Larger

Detection of Human Lactate Dehydrogenase B/LDHB by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and PC-3 human prostate cancer cell line. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Lactate Dehydrogenase B/LDHB antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

Lactate Dehydrogenase B/LDHB in Human Liver. Lactate Dehydrogenase B/LDHB was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry Lactate Dehydrogenase B/LDHB antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line. Lactate Dehydrogenase B/LDHB was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of Lactate Dehydrogenase B/LDHB antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Lactate Dehydrogenase B/LDHB in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205, filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Knockout Validated Western Blot Shows Human Lactate Dehydrogenase B/LDHB Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Lactate Dehydrogenase B/LDHB Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and LDHB knockout HEK293T cell line (KO). PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Lactate Dehydrogenase B/LDHB Monoclonal Antibody (Catalog # MAB9205) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitue at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lactate Dehydrogenase B/LDHB

LDHB is one of two subunits of Lactose Dehydrogenase which is formed from tetramer arrangements of the A and B subunits.  LDHB is part of the pyruvate fermentation to lactate pathway in which it catalyzes the conversion of L-lactate and NAD to pyruvate and NADH.  LDHA is widely expressed, with high expression in muscle and brain.  High expression in tumor cells correlates with low survival rates in various cancers including osteosarcoma, oral squamous cell carcinoma breast and lung cancers. Unlike Lactose Dehyrogenase A, LDHB mutations are not linked to any disease

Entrez Gene IDs
3945 (Human); 16832 (Mouse); 24534 (Rat)
Alternate Names
EC 1.1.1; EC 1.1.1.27; Epididymis Secretory Protein Li 281; HEL-S-281; Lactate Dehydrogenase B; LDH heart subunit; LDHB; LDH-B; LDHBD; LDH-H; L-lactate dehydrogenase B chain; Renal Carcinoma Antigen NY-REN-46; TRG-5

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Citations for Human Lactate Dehydrogenase B/LDHB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. AMPK&alpha loss promotes KRAS-mediated lung tumorigenesis
    Authors: M La Montagn, L Shi, P Magee, S Sahoo, M Fassan, M Garofalo
    Cell Death and Differentiation, 2021;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Transcriptional and Metabolic Dissection of ATRA-Induced Granulocytic Differentiation in NB4 Acute Promyelocytic Leukemia Cells
    Authors: J Albanesi, NI Noguera, C Banella, T Colangelo, E De Marinis, S Leone, O Palumbo, MT Voso, P Ascenzi, C Nervi, F Bianchi, A di Masi
    Cells, 2020;9(11):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human Lactate Dehydrogenase B/LDHB Antibody
By Anonymous on 11/24/2021
Application: WB Sample Tested: HeLa cells Species: Human