Human LIGHT/TNFSF14 PE-conjugated Antibody

Clone 115520 was used by HLDA to establish CD designation.
(4 citations)   
  • Species Reactivity
  • Specificity
    Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 115520
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
    Accession # O43557
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and 1 μg/mL Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 PE‑conjugated Monoclonal Antibody (Catalog # FAB664P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). Cells were gated on CD4+ cells using Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIGHT/TNFSF14

Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.

  • References:
    1. Mauri, D.N. et al. (1998) Immunity 8:21.
    2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
    3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
    4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.
  • Long Name:
    TNF Ligand Superfamily Member 14
  • Entrez Gene IDs:
    8740 (Human); 50930 (Mouse)
  • Alternate Names:
    CD258 antigen; CD258; delta transmembrane LIGHT; Herpes virus entry mediator ligand; Herpesvirus entry mediator ligand; HVEM-L; HVEMLherpesvirus entry mediator-ligand; ligand for herpesvirus entry mediator; LIGHTherpesvirus entry mediator A; LTg; TNFSF14; TR2; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; tumor necrosis factor receptor-like 2; tumor necrosis factor superfamily member LIGHT
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT.
    Authors: Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G
    Proc Natl Acad Sci U S A, 2014;111(52):E5688-96.
    Species: Human
    Sample Type: whole cells
    Application: Flow
  2. DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
    BMC Cancer, 2012;12(0):176.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Platelet-associated LIGHT (TNFSF14) mediates adhesion of platelets to human vascular endothelium.
    Authors: Celik S, Langer H, Stellos K, May AE, Shankar V, Kurz K, Katus HA, Gawaz MP, Dengler TJ
    Thromb. Haemost., 2007;98(4):798-805.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes.
    Authors: Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK
    Blood, 2006;108(3):928-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 28  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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