Detects human LIGHT/TNFSF14 in ELISAs. In sandwich immunoassays, no significant cross-reactivity or interference with recombinant human (rh) Fas Ligand, rhAPRIL, recombinant mouse TRANCE, rhTNF-alpha or rhTRAIL is observed.
Monoclonal Mouse IgG1 Clone # 115520
Protein A or G purified from ascites
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14 Asp74-Val240 Accession # O43557
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LIGHT/TNFSF14 in Human CD3+ T cells by Flow Cytometry. Human CD3+ T cells treated with 1 ng/mL PMA and 1 μg/mL Calcium Ionomycin for 36 hours were stained with Mouse Anti-Human LIGHT/TNFSF14 PE‑conjugated Monoclonal Antibody (Catalog # FAB664P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). Cells were gated on CD4+ cells using Mouse Anti-Human CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB3791A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Human LIGHT, also known as TNFSF14, is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma.
Mauri, D.N. et al. (1998) Immunity 8:21.
Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
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