Detection of LILRA4/CD85g/ILT7 in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human LILRA4/CD85g/ILT7 PE‑conjugated Monoclonal Antibody (Catalog # FAB6287P) and Goat Anti-Human DLEC/CLEC4C/BDCA‑2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1376A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Immunoglobulin-Like Receptor subfamily A member 4; also ILT-7 and CD85g) is a 35-38 kDa member of the Ig
superfamily of molecules. Mature LILRA4
is a 476 amino acid (aa) type I transmembrane glycoprotein that possesses four
C2-type Ig-like domains plus a nitrosylated tyrosine in its extracellular
region (aa 24-446). There is one
alternative start site at Met67 for human LILRA4, and rodent possesses no known
gene counterpart for primate LILRA4. LILRA4
reveals a very restricted pattern of expression, being found only on/in
microglia and immature plasmacytoid dendritic cells (pDC). On the cell surface, LILRA4 complexes with FceRI, and
appears to generally transmit downmodulating signals. Although controversial, one potential ligand
for LILRA4 has been identified and found to be BST2/CD317. Initially, it was suggested that LILRA4
served as a brake on type I interferon (IFN-a/b) production. In theory, viral nucleic acid detection by
TLR-7 or -9 in pDC would first induce IFN production, followed by
IFN-stimulated upregulation of BST2 on fibroblasts and endothelium, and ending
with BST2 binding to LILRA4 on pDC, downregulating the earlier virus-based
stimulation of IFN secretion. While this
may occur, an additional theory has been proposed for LILRA4 that is also based
on TLR-7 and -9 signaling. In this case,
LILRA4 is "activated" following TLR-7/-9 ligation and immature circulating pDC
will differentiate into APCs. But in the
absence of LILRA4 activation after TLR-7/-9 stimulation, pDC will differentiate
into fully functional type I interferon producing cells.
Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4
Entrez Gene IDs:
CD85 antigen-like family member G; CD85g antigen; CD85g; ILT7; ILT-7; ILT7MGC129598; Immunoglobulin-like transcript 7; leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member4; LILRA4; MGC129597
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