Human LILRA4/CD85g/ILT7 PE-conjugated Antibody

FAB6287P has been discontinued. View all LILRA4/CD85g/ILT7 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ILT7/CD85g in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LILRA5, ILT2, 3, 4, 5, or 6 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 656688
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ILT7/CD85g
    Glu24-Asn446
    Accession # P59901
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LILRA4/CD85g/ILT7 in Human Whole Blood Monocytes by Flow Cytometry. Human whole blood monocytes were stained with Mouse Anti-Human LILRA4/CD85g/ILT7 PE‑conjugated Monoclonal Antibody (Catalog # FAB6287P) and Goat Anti-Human DLEC/CLEC4C/BDCA‑2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1376A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LILRA4/CD85g/ILT7
LILRA4 (Leukocyte Immunoglobulin-Like Receptor subfamily A member 4; also ILT-7 and CD85g) is a 35-38 kDa member of the Ig superfamily of molecules. Mature LILRA4 is a 476 amino acid (aa) type I transmembrane glycoprotein that possesses four C2-type Ig-like domains plus a nitrosylated tyrosine in its extracellular region (aa 24-446). There is one alternative start site at Met67 for human LILRA4, and rodent possesses no known gene counterpart for primate LILRA4. LILRA4 reveals a very restricted pattern of expression, being found only on/in microglia and immature plasmacytoid dendritic cells (pDC). On the cell surface, LILRA4 complexes with FceRI, and appears to generally transmit downmodulating signals. Although controversial, one potential ligand for LILRA4 has been identified and found to be BST2/CD317. Initially, it was suggested that LILRA4 served as a brake on type I interferon (IFN-a/b) production. In theory, viral nucleic acid detection by TLR-7 or -9 in pDC would first induce IFN production, followed by IFN-stimulated upregulation of BST2 on fibroblasts and endothelium, and ending with BST2 binding to LILRA4 on pDC, downregulating the earlier virus-based stimulation of IFN secretion. While this may occur, an additional theory has been proposed for LILRA4 that is also based on TLR-7 and -9 signaling. In this case, LILRA4 is "activated" following TLR-7/-9 ligation and immature circulating pDC will differentiate into APCs. But in the absence of LILRA4 activation after TLR-7/-9 stimulation, pDC will differentiate into fully functional type I interferon producing cells.
  • Long Name:
    Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 4
  • Entrez Gene IDs:
    23547 (Human)
  • Alternate Names:
    CD85 antigen-like family member G; CD85g antigen; CD85g; ILT7; ILT-7; ILT7MGC129598; Immunoglobulin-like transcript 7; leukocyte immunoglobulin-like receptor subfamily A member 4; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 4; leukocyte immunoglobulin-like receptor, subfamily A (without TM domain), member4; LILRA4; MGC129597
Related Research Areas

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