Human Lipocalin-2/NGAL Quantikine QuicKit ELISA

Catalog # Availability Size / Price Qty
Human Lipocalin-2 Standard Curve ELISA
3 Images
Product Details

Human Lipocalin-2/NGAL Quantikine QuicKit ELISA Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (25 uL)
0.033 ng/mL
Assay Range
0.3 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Natural and recombinant human Lipocalin-2.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ QuicKit™ Human Lipocalin-2/NGAL Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Lipocalin-2 levels in cell culture supernates, serum, heparin plasma, saliva, and urine. It contains NS0-expressed recombinant human Lipocalin-2 and antibodies raised against the recombinant protein. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human Lipocalin-2.


Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 20 20 10 10
Mean (ng/mL) 1.01 6.05 1.11 6.46
Standard Deviation 0.025 0.261 0.058 0.379
CV% 2.5 4.3 5.2 5.9


The recovery of human Lipocalin-2 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum (n=)
Urine (n=2) 102 89-110


To assess linearity of the assay, samples containing and/or spiked with high concentrations of human Lipocalin-2 in various matrices were diluted with the calibrator diluent to produce samples with values within the dynamic range of the assay.
Human Linearity Curve

Scientific Data

Human Lipocalin-2 Standard Curve ELISA

Human Lipocalin-2 QuicKit ELISA Spiked Linearity Competitor Comparison Lipocalin-2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 89%-108% compared to 98%-129% for the top competitor.

Product Datasheets

You must select a language.


Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Lipocalin-2/NGAL

The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.

Long Name:
Neutrophil Gelatinase-associated Lipocalin
Entrez Gene IDs:
3934 (Human); 16819 (Mouse); 170496 (Rat); 102137149 (Cynomolgus Monkey)
Alternate Names:
24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Reviews for Human Lipocalin-2/NGAL Quantikine QuicKit ELISA

There are currently no reviews for this product. Be the first to review Human Lipocalin-2/NGAL Quantikine QuicKit ELISA and earn rewards!

Have you used Human Lipocalin-2/NGAL Quantikine QuicKit ELISA?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review