Human LYVE-1 APC-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human LYVE-1 in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 537028
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human LYVE-1
    Ser24-Thr238
    Accession # Q9Y5Y7
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LYVE-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human LYVE-1 APC-conjugated Monoclonal Antibody (Catalog # FAB20892A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LYVE-1
Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1 ‑ 3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9 ‑11).
  • References:
    1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
    2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
    3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
    4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
    5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
    6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
    7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
    8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
    9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
    10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
  • Long Name:
    Lymphatic Vessel Endothelial Hyaluronan Receptor 1
  • Entrez Gene IDs:
    10894 (Human); 114332 (Mouse); 293186 (Rat)
  • Alternate Names:
    cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Lymphatic vessels arise from specialized angioblasts within a venous niche.
    Authors: Nicenboim, J, Malkinson, G, Lupo, T, Asaf, L, Sela, Y, Mayseless, O, Gibbs-Bar, L, Senderovich, N, Hashimshony, T, Shin, M, Jerafi-Vider, A, Avraham-Davidi, I, Krupalnik, V, Hofi, R, Almog, G, Astin, J W, Golani, O, Ben-Dor, S, Crosier, P S, Herzog, W, Lawson, N D, Hanna, J H, Yanai, I, Yaniv, K
    Nature, 2015;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Identification of tumour-reactive lymphatic endothelial cells capable of inducing progression of gastric cancer.
    Authors: Tokumoto M, Tanaka H, Tauchi Y, Kasashima H, Kurata K, Yashiro M, Sakurai K, Toyokawa T, Kubo N, Amano R, Kimura K, Muguruma K, Maeda K, Ohira M, Hirakawa K
    Br J Cancer, 2015;113(7):1046-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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