Human M-CSF R Fluorescein-conjugated Antibody

(3 citations)   
  • Species Reactivity
  • Specificity
    Detects human M-CSF R in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) GM-CSF R alpha or rhGM‑CSF R beta.
  • Source
    Monoclonal Mouse IgG1 Clone # 61708
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human M-CSF R
    Ile20-Glu512 (Pro54Ala)
    Accession # P07333.2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of M‑CSF R in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human M‑CSF R Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB329F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin  alpha v beta 3 share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

  • References:
    1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
    2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
    3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
    4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
    5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
    6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
    7. Dai, X. et al. (2002) Blood 99:111.
    8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
    9. Li, M. et al. (2004) J. Neurochem. 91:623.
    10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
  • Long Name:
    Macrophage Colony Stimulating Factor Receptor
  • Entrez Gene IDs:
    1436 (Human)
  • Alternate Names:
    CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; MCSFR; M-CSFR; Proto-oncogene c-Fms
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Sample Type
  1. Colorectal cancer-derived microvesicles modulate differentiation of human monocytes to macrophages.
    Authors: Baj-Krzyworzeka M, Mytar B, Szatanek R, Surmiak M, Weglarczyk K, Baran J, Siedlar M
    J Transl Med, 2016;14(1):36.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Profiling of the three circulating monocyte subpopulations in human obesity.
    Authors: Devevre E, Renovato-Martins M, Clement K, Sautes-Fridman C, Cremer I, Poitou C
    J Immunol, 2015;194(8):3917-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Inhibition of RANK expression and osteoclastogenesis by TLRs and IFN-gamma in human osteoclast precursors.
    Authors: Ji JD, Park-Min KH, Shen Z, Fajardo RJ, Goldring SR, McHugh KP, Ivashkiv LB
    J. Immunol., 2009;183(11):7223-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 9  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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