Human mCherry Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB11041S Datasheet / COA / SDS
Recombinant Monoclonal Antibody
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Product Details
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Human mCherry Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Detects human mCherry in direct ELISAs.
Recombinant Monoclonal Rabbit IgG Clone # 2783C
Protein A or G purified from cell culture supernatant
Human mCherry synthetic peptide
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)


Recommended Concentration
Flow Cytometry
Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment was HEK293 human embryonic kidney cell line transfected with mCherry.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: mCherry

mCherry is a member of the mFruits family of monomeric red fluorescent proteins.  It was derived from DsRed of Discoscoma sea anemones.  mFruit are second-generation monomeric red fluorescent proteins that have improved brightness and photostability compared to first-generation proteins.  The gene for mCherry is 711bp long and the protein is made up of 236 residues with a mass of 76.722 kDa.  mCherry is used in fluorescence microscopy as an intracellular probe where constitutive gene expression is desired and other experimental approaches require coordinated control of multiple genes. 

References Shaner, NC., Campbell, R E., Steinbach, P A., Giepmans B N G., Palmer A E., Tsien, R Y. "Improved Monomeric Red, Orange and Yellow Fluorescent Proteins Derived from Discomsoma Sp. Red Fluorescent Protein" Nature Biotechnology. 22(12):  1567-1572. 2004 Nov 21. Shu, X., Shaner N C., Yarbrough, CA., Tsien, R Y., Remington S J. "Novel Chromophores and Buried Charges Control Color in mFruits" Biochemistry. 45(32):9639-9647. 2006 Aug. Gebhardt, M J., Jacobson, R K., Shuman, H. "Seeing Red; The Development of pON mCherry, a Broad-host Range Constitutive Expression Plasmid for Gram-negative Bacteria" POS ONE. 12(3):e173116. 2017 Mar 3.
Long Name
Alternate Names
DSRED; mCherry; red fluorescent protein mCherry; Red Fluoroscent Protein

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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