Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2783C
View all mCherry Primary Antibodies »

Product Specifications

Immunogen

mCherry synthetic peptide

Specificity

Detects mCherry in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for mCherry Antibody

Detection of mCherry by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mVISTA. PVDF membrane was probed with 0.25 µg/mL of Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for mCherry at approximately 30-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

mCherry in HEK293 Human Cell Line Transfected with mCherry.

mCherry was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with mCherry using Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of mCherry in HEK293 Human Cell Line transfected with mCherry by Flow Cytometry.

HEK293 human cell line transfected with mCherry (filled histogram) or EGFP (open histogram) was stained with Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041), followed by Allophycocyanin-conjugated Anti-Rabbit IgG F(ab')2Secondary Antibody (F0111). Gates were set based on isotype control antibody (MAB1050, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Detection of mCherry by Simple WesternTM.

Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with mVISTA, loaded at 0.2 mg/mL. Specific bands were detected for mCherry at approximately 36 and 110 kDa (as indicated) using 25 µg/mL of Rabbit Anti-mCherry Monoclonal Antibody (Catalog # MAB11041). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for mCherry Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with mCherry

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mCherry

Simple Western

25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with mVISTA

Western Blot

0.25 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with mVISTA

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mCherry

mCherry is a member of the mFruits family of monomeric red fluorescent proteins. It was derived from DsRed of Discoscoma sea anemones. mFruit are second-generation monomeric red fluorescent proteins that have improved brightness and photostability compared to first-generation proteins. The gene for mCherry is 711bp long and the protein is made up of 236 residues with a mass of 76.722 kDa. mCherry is used in fluorescence microscopy as an intracellular probe where constitutive gene expression is desired and other experimental approaches require coordinated control of multiple genes.

References

  1. Shaner, N.C. Campbell, R.E. Steinbach, P.A. Giepmans B.N.G. Palmer A.E. Tsien, R Y. "Improved Monomeric Red, Orange and Yellow Fluorescent Proteins Derived from Discomsoma Sp. Red Fluorescent Protein" Nature Biotechnology. 22(12):1567. 2004 Nov. 21.
  2. Shu, X. Shaner N C. Yarbrough, C.A. Tsien, R Y. Remington S.J. "Novel Chromophores and Buried Charges Control Color in mFruits" Biochemistry. 45(32):9639. 2006 Aug.
  3. Gebhardt, M.J. Jacobson, R.K. Shuman, H. "Seeing Red; The Development of pON mCherry, a Broad-host Range Constitutive Expression Plasmid for Gram-negative Bacteria" POS ONE. 12(3):e173116. 2017 Mar. 3.

Long Name

mCherry

Alternate Names

DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

Additional mCherry Products

Product Documents for mCherry Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for mCherry Antibody

For research use only

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Protocols

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