Human MDC1 Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB6497G Datasheet / COA / SDS

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FAB6497G-100UG

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Human MDC1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MDC1 in Western blots.

Source

Monoclonal Rat IgG2a Clone # 398636

Purification

Protein A or G purified

Immunogen

E.coli-derived recombinant human MDC1

Met1-Glu335
Accession # Q14676

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MDC1

Mediator of DNA damage checkpoint protein 1 (MDC1), also known as nuclear factor with BRCT domains protein 1 (NFBD1), is a 250 kDa nuclear protein that is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. Human MDC1 is 2089 amino acids (aa) in length and contains a forkhead-associated motif at its N terminus, two BRCT motifs at its C terminus, and 13 internal repetitions of a 41 aa sequence enriched with seine, threonine, and proline residues. Splicing variants produce five isoforms of MDC1. Human MDC1 shares 49% aa sequence identity with mouse MDC1. MDC1 is highly expressed in testis.

Long Name

Mediator of DNA-damage checkpoint 1

Entrez Gene IDs

9656 (Human)

Alternate Names

DKFZp781A0122; Em:AB023051.5; KIAA0170MGC166888; MDC1; mediator of DNA damage checkpoint 1; mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; NFBD1; NFBD1homologue to Drosophila photoreceptor protein calphotin; Nuclear factor with BRCT domains 1