Detection of Human MDC1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Human MDC1 Monoclonal Antibody (Catalog # MAB6497) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MDC1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mediator of DNA damage
checkpoint protein 1 (MDC1), also known as nuclear
factor with BRCT domains protein 1 (NFBD1), is a 250 kDa nuclear protein that is required to activate
the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA
damage. Human MDC1 is 2089 amino acids
(aa) in length and contains a forkhead-associated motif at its N terminus, two
BRCT motifs at its C terminus, and 13 internal repetitions of a 41 aa sequence
enriched with seine, threonine, and proline residues. Splicing variants produce five isoforms of MDC1. Human MDC1 shares 49% aa
sequence identity with mouse MDC1. MDC1 is highly expressed in testis.
Mediator of DNA-damage checkpoint 1
Entrez Gene IDs:
DKFZp781A0122; Em:AB023051.5; KIAA0170MGC166888; mediator of DNA damage checkpoint 1; mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; NFBD1; NFBD1homologue to Drosophila photoreceptor protein calphotin; Nuclear factor with BRCT domains 1
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