Human MDC1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human MDC1 by Western Blot.
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Product Details
Supplemental Products

Human MDC1 Antibody Summary

Species Reactivity
Detects human MDC1 in Western blots.
Monoclonal Rat IgG2A Clone # 398636
Protein A or G purified from hybridoma culture supernatant

E.coli-derived recombinant human MDC1

Accession # Q14676
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human MDC1 antibody by Western Blot. View Larger

Detection of Human MDC1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Human MDC1 Monoclonal Antibody (Catalog # MAB6497) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MDC1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MDC1

Mediator of DNA damage checkpoint protein 1 (MDC1), also known as nuclear factor with BRCT domains protein 1 (NFBD1), is a 250 kDa nuclear protein that is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage.  Human MDC1 is 2089 amino acids (aa) in length and contains a forkhead-associated motif at its N terminus, two BRCT motifs at its C terminus, and 13 internal repetitions of a 41 aa sequence enriched with seine, threonine, and proline residues.  Splicing variants produce five isoforms of MDC1.  Human MDC1 shares 49% aa sequence identity with mouse MDC1.  MDC1 is highly expressed in testis.

Long Name
Mediator of DNA-damage checkpoint 1
Entrez Gene IDs
9656 (Human)
Alternate Names
DKFZp781A0122; Em:AB023051.5; KIAA0170MGC166888; MDC1; mediator of DNA damage checkpoint 1; mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; NFBD1; NFBD1homologue to Drosophila photoreceptor protein calphotin; Nuclear factor with BRCT domains 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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