Human MDC1 Antibody Summary
E.coli-derived recombinant human MDC1
Accession # Q14676
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human MDC1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF Membrane was probed with 2 µg/mL of Human MDC1 Monoclonal Antibody (Catalog # MAB6497) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for MDC1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mediator of DNA damage checkpoint protein 1 (MDC1), also known as nuclear factor with BRCT domains protein 1 (NFBD1), is a 250 kDa nuclear protein that is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. Human MDC1 is 2089 amino acids (aa) in length and contains a forkhead-associated motif at its N terminus, two BRCT motifs at its C terminus, and 13 internal repetitions of a 41 aa sequence enriched with seine, threonine, and proline residues. Splicing variants produce five isoforms of MDC1. Human MDC1 shares 49% aa sequence identity with mouse MDC1. MDC1 is highly expressed in testis.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human MDC1 Antibody
There are currently no reviews for this product. Be the first to review Human MDC1 Antibody and earn rewards!
Have you used Human MDC1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image