Human Melanotransferrin/CD228 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB81751N Datasheet / COA / SDS
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Human Melanotransferrin/CD228 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Detects human Melanotransferrin/CD228 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 893416
HEK293 human embryonic kidney cell line transfected with human Melanotransferrin/CD228
Accession # P08582
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)


Recommended Concentration
Flow Cytometry
0.25-1 µg/106 cells
A431 human epithelial carcinoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Melanotransferrin/CD228

Melanotransferrin, also known as MTF, CD228, Melanoma-associated Antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is 738 amino acids (aa). Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Entrez Gene IDs
4241 (Human); 30060 (Mouse); 288038 (Rat)
Alternate Names
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5; CD228 antigen; CD228; FLJ38863; MAP97; MAP97Melanoma-associated antigen p97; Melanotransferrin; membrane-bound transferrin-like protein; MFI2; MGC4856; MTF; MTF1; p97

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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