Human Melanotransferrin/CD228 Antibody

Catalog # Availability Size / Price Qty
Detection of Melanotransferrin/CD228 in A431 Human Cell Line by Flow Cytometry.
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Product Details
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Human Melanotransferrin/CD228 Antibody Summary

Species Reactivity
Detects human Melanotransferrin/CD228 in direct ELISAs.
Monoclonal Mouse IgG1 Clone # 893416
Protein A or G purified from hybridoma culture supernatant
HEK293 human embryonic kidney cell line transfected with human Melanotransferrin/CD228
Accession # P08582
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Melanotransferrin/CD228 antibody in A431 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Melanotransferrin/CD228 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human Melanotransferrin/CD228 Monoclonal Antibody (Catalog # MAB81751, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Melanotransferrin/CD228

Melanotransferrin, also known as MTF, CD228, Melanoma-associated Antigen p97, MAP97 and MFI2, is a 90-97 kDa sialoglycoprotein member of the transferrin family. Unlike other transferrins, which are secreted, MTF is usually found tethered to the cell membrane by a glycosyl phosphatidyl inositol anchor, with only small amounts of soluble protein detected. MTF is highly expressed on melanoma cells, and at lower levels in salivary gland, pancreas, kidney and testis. Like other transferrins, MTF is an iron-binding protein, and may play roles in cellular proliferation, tumorigenesis, metastasis and migration. Full-length human, mouse and rat MTF is 738 amino acids (aa). Over aa 1-711, human MTF shares 85 and 86% aa identity with mouse and rat MTF, respectively.

Entrez Gene IDs
4241 (Human); 30060 (Mouse); 288038 (Rat)
Alternate Names
antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and96.5; CD228 antigen; CD228; FLJ38863; MAP97; MAP97Melanoma-associated antigen p97; Melanotransferrin; membrane-bound transferrin-like protein; MFI2; MGC4856; MTF; MTF1; p97

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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