Human Mer Alexa Fluor® 700-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB8912N
Human Mer Alexa Fluor® 700-conjugated Antibody in Data
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Human Mer Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Axl, rhDtk, or recombinant mouse Mer is observed.
Source
Monoclonal Mouse IgG2B Clone # 125518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Met1-Ala499
Accession # AAB60430
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Mer in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Mer in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB8912N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Mer in U937 Human Cell Line by Flow Cytometry. View Larger

Detection of Mer in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB8912N, filled histogram) or isotype control antibody (Catalog # IC0041N, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mer

Axl (also known as Ufo, Ark), Dtk (also known as Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

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