Human Mer Antibody (125518)

R&D Systems | Catalog # MAB8912

R&D Systems
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, ELISA Capture (Matched Antibody Pair), Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation, Activation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 125518
View all Mer Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Mer
Met1-Ala499
Accession # AAB60430

Specificity

Detects human Mer in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Axl, rhDtk, or recombinant mouse Mer is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Mer Antibody (125518)

Detection of Mer antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of Mer in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Detection of Mer antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of Mer in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Mer Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

Mer Specificity is Shown by Flow Cytometry in Knockout Cell Line.

Mer knockout HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the Mer knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Human Human Mer Antibody by Flow Cytometry

Detection of Human Human Mer Antibody by Flow Cytometry

MR766 infection of unstimulated and decidualized T-HESC cell line.(a) T-HESC (left) and decidualized (d) T-HESC (right) were stained with ZIKV anti-dsRNA or E protein mAbs whereas the nuclei were stained with Hoechst. (b) Surface expression of AXL (red) and MER (blue) in T-HESC (left) and dT-HESC (right) was determined in uninfected cells by flow cytometry. The histograms of one experiment representative of 3 independently performed are shown. Double immunostaining for ZIKV E protein and calreticulin (c) or dsRNA and vimentin (d) in dT-HESC either uninfected or infected with MR766 at 72 h post-infection; Hoechst was used to stain nuclei. Arrows indicate localization of E protein in areas occupied by calreticulin. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28281680), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mer by Flow Cytometry

Detection of Mer by Flow Cytometry

Expression of Mer on dendritic cell populations from the peripheral blood of normal individuals. (A) Gating strategy for the identification of dendritic cells subsets. (B) Expression of Mer on the surface of CD1c+ myeloid dendritic cells subpopulations from the peripheral blood of a representative normal individual. (C) Expression of Mer on the surface of pDCs on myeloid dendritic cells subpopulations from the peripheral blood of a representative normal individual. (D) Expression of Mer on dendritic subsets of normal individuals (n = 15). Horizontal bars represent mean values. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/24650765), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human Mer ELISA Standard Curve

Human Mer ELISA Standard Curve

Recombinant Human Mer Fc Chimera (Catalog # 891-MR) was serially diluted and captured by Mouse Anti-Human Mer Monoclonal Antibody (Catalog # MAB8912) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Mer Antigen Affinity-purified Polyclonal Antibody (Catalog # AF891) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human Mer Antibody (125518)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line and U937 human histiocytic lymphoma cell line

Knockout Validated

0.25 µg/106 cells
Sample: Mer is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in Mer knockout HepG2 cell line.

Human Mer Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Mer Biotinylated Antibody (Catalog # BAF891)
  • Standard: Recombinant Human Mer Fc Chimera Protein, CF (Catalog # 891-MR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 4.7 using MAB8912 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Recent studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions.

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

AAB60430

Additional Mer Products

Product Documents for Human Mer Antibody (125518)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mer Antibody (125518)

For research use only

Citations for Human Mer Antibody (125518)

Customer Reviews for Human Mer Antibody (125518) (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Detection of MerTK in M2c macrophages
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: M2c macrophages
    Species: Human
    Verified Customer | Posted 06/20/2026
    Detection of MerTK in M2c macrophages. The cells were incubated with 2 μl of the human MerTK antibody (Red) or isotype control (blue) for 30 minutes on ice, followed by a secondary a-mouse IgG APC
    The antibody was used to detect MerTK in M2c macrophages using 2 μl of the human MerTK antibody or isotype control for 30 minutes on ice, followed by a secondary a-mouse IgG APC at 1:300
    Human Mer Antibody (125518) MAB8912
  • Name: Sophie Lin
    Verified Customer | Posted 04/26/2017
  • Human Mer Antibody
    Name: sothy yi
    Application: Functional Assay
    Sample Tested: NCI-H1299
    Species: Human
    Verified Customer | Posted 06/07/2016
    Human Mer Antibody (125518) MAB8912

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