Human MINA Alexa Fluor® 750-conjugated Antibody

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R&D Systems Antibodies
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Product Details

Human MINA Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.
Monoclonal Mouse IgG1 Clone # 753002
E. coli-derived recombinant human MINA
Accession # Q8IUF8
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MINA

MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145‑465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.

Long Name
Myc-induced Nuclear Antigen
Entrez Gene IDs
84864 (Human); 67014 (Mouse); 266670 (Rat)
Alternate Names
DKFZp762O1912; FLJ14393; MDIG; MDIGDKFZp762O1912; MINA; Mina53; MINA53mdig; mineral dust induced gene protein; Mineral dust-induced gene protein; MYC induced nuclear antigen; MYC-induced nuclear antigen; myc-induced nuclear antigen, 53 kDa; NO52; Nucleolar protein 52

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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