Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 753002
View all MINA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MINA
Met1-Gly192
Accession # Q8IUF8

Specificity

Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human MINA Antibody

Detection of Human MINA antibody by Western Blot.

Detection of Human MINA by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JAR human choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MINA Monoclonal Antibody (Catalog # MAB7476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MINA at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of MINA antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of MINA in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human MINA Monoclonal Antibody (Catalog # MAB7476, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human MINA Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and JAR human choriocarcinoma cell line

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MINA

MINA (myc-induced nuclear antigen; also Mina53) is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, occasional squamous epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R and IL-4. It may exert its regulatory activity through an intrinsic demethylase function. Mouse MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 51-363) that contains a JmjC domain (aa 139-271). There are two potential isoform variants that contain either a 12 aa substitution for aa 145‑465, or a 15 aa substitution for aa 228-465. Over aa 2-192, mouse MINA shares 92% and 82% aa sequence identity with rat and human MINA, respectively.

Long Name

Myc-induced Nuclear Antigen

Alternate Names

MDIG, Mina53, NO52

Entrez Gene IDs

84864 (Human); 67014 (Mouse); 266670 (Rat)

Gene Symbol

RIOX2

UniProt

Q8IUF8 (Human)

Additional MINA Products

Product Documents for Human MINA Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MINA Antibody

For research use only

Related Research Areas

Cancer Biomarkers

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