Detection of Human and Mouse Adiponutrin/PNPLA3 by Western Blot.
Western blot shows lysates of BG01V human embryonic stem cells, mouse liver tissue, human brain tissue, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Adiponutrin/PNPLA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/PNPLA3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse adiponutrin (also patatin-like phospholipase domain-containing protein 3) is a 45-48 kDa member of the adiponutrin family of phospholipase A2 enzymes. It is expressed in white and brown adipocytes, hepatocytes and brain. Adiponutrin is reduced in amount during fasting, and appears to act as an acute sensor of the body energy stores. Based on other family members, mouse adiponutrin is a type II transmembrane protein that is 413 amino acids (aa) in length. It contains one patatin domain (aa 10-179) that is associated with enzyme activity. There appear to be three splice variants. One shows a 69 aa substitution for aa 384-413, a second shows a deletion of aa 385-413, while a third shows a three aa substitution for aa 384-413. Over aa 160-327, mouse adiponutrin shares 84% and 62% aa identity with rat and human adiponutrin, respectively; and over aa 161-229, mouse adiponutrin shares 90% aa identity with human adiponutrin.
Patanin-like Phospholipase Domain-containing Protein 3
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