Human/Mouse Adiponutrin/PNPLA3 Antibody

AF5179 has been discontinued. View all Adiponutrin/PNPLA3 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Adiponutrin/PNPLA3 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse Adiponutrin/PNPLA3
    Arg160-Arg327
    Accession # Q91WW7
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Adiponutrin/PNPLA3 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells, mouse liver tissue, human brain tissue, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Adiponutrin/PNPLA3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5179) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Adiponutrin/PNPLA3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponutrin/PNPLA3

Mouse adiponutrin (also patatin-like phospholipase domain-containing protein 3) is a 45-48 kDa member of the adiponutrin family of phospholipase A2 enzymes. It is expressed in white and brown adipocytes, hepatocytes and brain. Adiponutrin is reduced in amount during fasting, and appears to act as an acute sensor of the body energy stores. Based on other family members, mouse adiponutrin is a type II transmembrane protein that is 413 amino acids (aa) in length. It contains one patatin domain (aa 10-179) that is associated with enzyme activity. There appear to be three splice variants. One shows a 69 aa substitution for aa 384-413, a second shows a deletion of aa 385-413, while a third shows a three aa substitution for aa 384-413. Over aa 160-327, mouse adiponutrin shares 84% and 62% aa identity with rat and human adiponutrin, respectively; and over aa 161-229, mouse adiponutrin shares 90% aa identity with human adiponutrin.

  • Long Name:
    Patanin-like Phospholipase Domain-containing Protein 3
  • Entrez Gene IDs:
    80339 (Human); 362972 (Rat)
  • Alternate Names:
    Acylglycerol O-acyltransferase; Adiponutrin; ADPN; ADPNdJ796I17.1; C22orf20; Calcium-independent phospholipase A2-epsilon; chromosome 22 open reading frame 20; EC 2.3.1.-; EC 2.7.7.56; EC 3.1.1.3; EC 4.2.3.4; FLJ22012; iPLA(2)epsilon; iPLA2-epsilon; patatin-like phospholipase domain containing 3; patatin-like phospholipase domain-containing protein 3; PNPLA3
Related Research Areas

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