Human/Mouse BMP‑8b Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF6305AFP488

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Specificity

Detects mouse BMP-8b in direct ELISAs and human and mouse in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) BMP-8a and less than 1% cross‑reactivity with recombinant human (rh) BMP‑3b, rhBMP‑10, and rm BMP‑10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human/Mouse BMP‑8b Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BMP-8b

BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.

Long Name

Bone Morphogenetic Protein 8b

Alternate Names

OP-3

Entrez Gene IDs

656 (Human); 12164 (Mouse)

Gene Symbol

BMP8B

UniProt

Additional BMP-8b Products

Product Documents for Human/Mouse BMP‑8b Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse BMP‑8b Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

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