BMP-8b Antibody

R&D Systems | Catalog # AF6305

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BMP-8b Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse BMP-8b
Thr261-His399
Accession # NP_031585

Specificity

Detects mouse BMP-8b in direct ELISAs and human and mouse in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) BMP-8a and less than 1% cross‑reactivity with recombinant human (rh) BMP‑3b, rhBMP‑10, and rm BMP‑10 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for BMP-8b Antibody

Detection of Mouse BMP-8b antibody by Western Blot.

Detection of Mouse BMP‑8b by Western Blot.

Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse BMP-8b Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6305) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pro-BMP-8b at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for BMP-8b Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse brain tissue

Reviewed Applications

Read 1 review rated 4 using AF6305 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-8b

BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.

Long Name

Bone Morphogenetic Protein 8b

Alternate Names

OP-3

Entrez Gene IDs

656 (Human); 12164 (Mouse)

Gene Symbol

BMP8B

UniProt

NP_031585

Additional BMP-8b Products

Product Documents for BMP-8b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for BMP-8b Antibody

For research use only

Related Research Areas

BMP Family

Customer Reviews for BMP-8b Antibody (1)

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  • Human/Mouse BMP-8b Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Liver tissue
    Species: Human and Mouse
    Verified Customer | Posted 10/05/2020
    The figures was used to stain with IF BMP8B in human and murine FFPE liver slides with IF. Details of the figures (Fig 1 and Extended Figure 1) and experimental conditions can be found in the published paper: https://www.nature.com/articles/s42255-020-0214-9 where it is shown that BMP8B is expressed in acute and chronic liver disease by hepatocytes (CK18+/Alb+) and Activated Stellate Cells (aSMA+) both in humans and mice.
    BMP-8b Antibody AF6305

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