Detection of CELSR2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse CELSR2 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6739G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2; also cadherin family member 10/CDHF10, Flamingo1 and EGFL2) is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa identity with mouse CELSR2.
Cadherin, EGF LAG Seven-pass G-type Receptor 2
Entrez Gene IDs:
1952 (Human); 53883 (Mouse); 83465 (Rat)
cadherin EGF LAG seven-pass G-type receptor 2; Cadherin family member 10; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila); CDHF10; CDHF10FLJ34118; CELSR2; EGFL2; EGFL2FLJ45143; EGF-like protein 2; EGF-like-domain, multiple 2; epidermal growth factor-like 2; Epidermal growth factor-like protein 2; Flamingo homolog 3; Flamingo1; FLJ45845; KIAA0279FLJ42737; MEGF3; MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog; Multiple EGF-like domains protein 3; multiple epidermal growth factor-like domains 3; Multiple epidermal growth factor-like domains protein 3
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