|Detection of CELSR2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse CELSR2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6739A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.|
CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2), also known as Cadherin Family Member 10/CDHF10, Flamingo1 and EGFL2, is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids (aa) in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa sequence identity with mouse CELSR2.
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