Human/Mouse CELSR2 APC-conjugated Antibody

FAB6739A has been discontinued. View all CELSR2 products.
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human CELSR2 in direct ELISAs and Western blots. Detects human and mouse CELSR2 in flow cytometry.  In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) CELSR1 and rhCELSR3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CELSR2
    Cys51-Phe231
    Accession # Q9HCU4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CELSR2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse CELSR2 APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6739A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CELSR2

CELSR2 (Cadherin EGF LAG seven-pass G-type receptor 2), also known as Cadherin Family Member 10/CDHF10, Flamingo1 and EGFL2, is a 300-330 kDa member of the LN‑7TM subfamily, GPCR 2 family of proteins. It is expressed on neurons, breast epithelium, Sertoli cells and germ cells, and through homophilic interactions, serves as either an adhesion or guidance molecule. Mature human CELSR2 is 2892 amino acids (aa) in length (aa 32-2923). It is a highly complex 7-transmembrane protein that contains a 2349 aa extended N-terminal extracellular region (aa 32-2380) plus a 310 aa C-terminal cytoplasmic domain. The N-terminal region contains nine consecutive cadherin domains (aa 182-1146) followed by a mixture of seven EGF-like and three laminin-like domains. There is a proteolytic cleavage site between Met2356-Thr2357 that generates a 250 kDa soluble fragment and a (mature) 60-65 kDa transmembrane segment that may reside on the cell membrane. Over aa 51‑231, human CELSR2 shares 93% aa sequence identity with mouse CELSR2.

  • Long Name:
    Cadherin, EGF LAG Seven-pass G-type Receptor 2
  • Entrez Gene IDs:
    1952 (Human); 53883 (Mouse); 83465 (Rat)
  • Alternate Names:
    cadherin EGF LAG seven-pass G-type receptor 2; Cadherin family member 10; cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila); CDHF10; CDHF10FLJ34118; CELSR2; EGFL2; EGFL2FLJ45143; EGF-like protein 2; EGF-like-domain, multiple 2; epidermal growth factor-like 2; Epidermal growth factor-like protein 2; Flamingo homolog 3; Flamingo1; FLJ45845; KIAA0279FLJ42737; MEGF3; MEGF3cadherin, EGF LAG seven-pass G-type receptor 2, flamingo (Drosophila) homolog; Multiple EGF-like domains protein 3; multiple epidermal growth factor-like domains 3; Multiple epidermal growth factor-like domains protein 3
Related Research Areas

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