Human/Mouse DNMT3A Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: DNMT3A

DNMT3A (DNA [cytosine-5]-methyltransferase 3A) is a 130 kDa member of the C5-methlytransferase family of molecules.  It is expressed both during development and in virtually all adult tissues except for small intestine.  DNMT3A is a nuclear protein that is found associated with heterochromatin.  It possesses DNA methyltransferase activity, and acts in a complex with DNMT3L in embryonic stem cells, forming a (3A)2:(3L)2 heterotetramer.  It also interacts with SETDB1 to form a transcriptional repressor.  Human DNMT3A is 912 amino acids (aa) in length.  It contains one ProTrpTrpPro domain (aa 292-350) and one ADD-type zinc finger region (aa 494-586).  There is one 100 kDa splice variant termed DNMT3a2 that contains an alternative start site at Met224, and preferentially interacts with euchromatin.  Other potential splice variants show a 16 aa substitution for aa 151-912, and a 24 aa substitution for aa 1-213.  Over aa 353-486, human DNMT3A shares 96% aa identity with mouse DNMT3A.  

Product Datasheets

Related Research Areas

• Adult Neurogenesis
• DNA Modifying Enzymes
• Epigenetics
• Hematopoietic Stem Cell Transcription Factors and Regulators
• Methyltransferases and Demethylases
• Stem Cell Epigenetics