GDPD2 in MC3T3‑E1 Mouse Cell Line.
GDPD2 was detected in immersion fixed MC3T3‑E1 mouse preosteoblast cell line using Mouse Anti-Human GDPD2 Monoclonal Antibody (Catalog # MAB7026) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Glycerophosphodiester phosphodiesterase 3 (GDPD2), also known as GDE3, is a 65 kDa 7TM cell surface protein that is highly expressed in bone and spleen. GDPD2 promotes osteoblast differentiation and induces changes in the actin cytoskeleton. Its 225 amino acid (aa) N-terminal ECD contains a domain that is homologous to bacterial glycerophosphodiester phosphodiesterases. Human GDPD2 shares 85% aa sequence identity with mouse and rat GDPD2.
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