Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody

R&D Systems | Catalog # IC1935N

R&D Systems
Discontinued Product
IC1935N has been discontinued. View all HIF-1 alpha/HIF1A products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Western Blot, Flow Cytometry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 241812
View all HIF-1 alpha/HIF1A Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HIF-1 alpha
Arg575-Asn826
Accession # Q16665.1

Specificity

Recognizes human and mouse HIF-1 alpha in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody

Detection of HIF-1a antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of HIF‑1 alpha in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line treated with CoCl2was stained with Mouse Anti-Human/Mouse HIF-1a Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1935N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: MCF‑7 human breast cancer cell line, treated with CoCl2, and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 5 using IC1935N in the following applications:

Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HIF-1 alpha/HIF1A

The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha ) is the regulated member of the transcription factor heterodimer HIF-1. HIF-1 binds to hypoxia-response elements (HREs) in the promoters of many genes involved in adapting to an environment of insufficient oxygen or hypoxia. Hypoxic tissue environments occur in vascular and pulmonary diseases as well as cancer, which illustrates the broad impact of gene regulation by HIF-1 alpha.

Long Name

Hypoxia Inducible Factor 1 Subunit Alpha

Alternate Names

BHLHE78, HIF 1A, HIF-1a, HIF1 alpha, HIF1A, MOP1, PASD8

Entrez Gene IDs

3091 (Human); 15251 (Mouse); 29560 (Rat)

Gene Symbol

HIF1A

UniProt

Q16665.1

Additional HIF-1 alpha/HIF1A Products

Product Documents for Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Citations for Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody

Customer Reviews (1)

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  • Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Whole blood B cells
    Species: Human
    Verified Customer | Posted 09/28/2021
    Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700‑conjugated Antibody IC1935N

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