Human/Mouse IL‑19 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB2915AFP488

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 350114
View all IL-19 Primary Antibodies »

Product Specifications

Specificity

Detects human and mouse IL-19 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Human/Mouse IL‑19 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IL-19

Interleukin 19 (IL-19) is a member of the IL-10 family of cytokines (1). The IL-10 family is a class II alpha -helical collection of cytokines that contains two groups, a viral homolog and a cellular homolog group. Within the cellular homolog group, there are two additional groupings, one which uses IL-10 R2 as a signal transducing receptor (IL-10, IL-22 and IL-26), and one which uses IL-20 R2 as a signal transducing receptor (IL-19, IL-20 and IL-24) (2 - 4). Mouse IL-19 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature region (5). It is secreted as a glycosylated monomer, 35 - 45 kDa in size (2, 6, 7). IL-19 is unusual in that it contains seven amphipathic helices (2, 4, 8). Mature mouse IL-19 shares 69% aa sequence identity with the mature human IL-19, and 85% and 68% aa identity with rat and canine IL-19, respectively. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells (5). IL-19 expression is limited to activated keratinocytes and monocytes, with a possible contribution from B cells (6, 9, 10). IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R2) (3, 4, 11, 12). This receptor complex is also shared by IL-20 and IL-24. Notably, IL-19 is reported to actually bind to IL-20 R2, which is generally considered to be only the signal transducing receptor subunit (7, 13). Functionally, it has been reported that IL‑19 both will and will not induce IL-6 and TNF production by monocytes (5, 14). It does, however, seem to drive T-helper cell differentiation towards a Th2 response, inducing both IL-10 and production of itself (5, 14, 15).

References

  1. Fickenscher, H. et al. (2002) Trends Immunol. 23:89.  
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. 
  3. Zdanov (2004) Curr. Pharm. Des. 10:3873. 
  4. Gallagher, G. et al. (2004) Int. Immunopathol. 4:615.  
  5. Laio, Y.-C. et al. (2002) J. Immunol. 169:4288. 
  6. Gallagher, G. et al. (2000) Genes Immun. 1:442. 
  7. Pletnev, S. et al. (2003) Biochemistry 42:12617. 
  8. Chang, C. et al. (2003) J. Biol. Chem. 278:3308.
  9. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.
  10. Wolk, K. et al. (2002) J. Immunol. 168:5397.
  11. Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
  12. Parrish-Novak, J. et al. (2002) J. Biol. Chem. 277:47517
  13. Preimel, D. and H. Sticht (2004) J. Mol. Model. 10:290.
  14. Jordan, W.J. et al. (2005) Eur. J. Immunol. 35:1576.
  15. Laio, S.-C. et al. (2004) J. Immunol. 173:6712.

Long Name

Interleukin 19

Alternate Names

IL19

Entrez Gene IDs

29949 (Human); 329244 (Mouse)

Gene Symbol

IL19

UniProt

Q8CJ70

Additional IL-19 Products

Product Documents for Human/Mouse IL‑19 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse IL‑19 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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