Human/Mouse MMP‑24/MT5‑MMP PE‑conjugated Antibody

R&D Systems | Catalog # FAB924P

R&D Systems
Discontinued Product
FAB924P has been discontinued. View all MMP-24/MT5-MMP products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 143908
View all MMP-24/MT5-MMP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse MMP‑24/MT5-MMP
Arg125-Gln296
Accession # Q9R0S2

Specificity

Detects mouse MMP‑24/MT5-MMP in direct ELISAs and detects human and mouse MMP‑24/MT5-MMP in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-9, and the catalytic domains of recombinant human MMP‑14, 15, and 16 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse MMP‑24/MT5‑MMP PE‑conjugated Antibody

Detection of MMP-24/MT5-MMP antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of MMP‑24/MT5‑MMP in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Human/Mouse MMP-24/MT5-MMP PE-conjugated Monoclonal Antibody (Catalog # FAB924P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of MMP-24/MT5-MMP antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of MMP‑24/MT5‑MMP in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Rat Anti-Human/Mouse MMP-24/MT5-MMP PE-conjugated Monoclonal Antibody (Catalog # FAB924P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse MMP‑24/MT5‑MMP PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: bEnd.3 mouse endothelioma cell line and A172 human glioblastoma cell line

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: MMP-24/MT5-MMP

MMP-24, also known as MT5-MMP, is a 62-65 kDa member of the M10 metallopeptidase family, a subfamily of MMPs. It is a type I transmembrane glycoprotein that is converted into a 58 kDa active form (amino acids [aa] 129-618) following removal of its propeptide. Proteolytic cleavage after Arg554 generates a 52 kDa active, soluble form that undergoes further cleavage in to 27-34 kDa fragment. MMP-24 has restricted expression, being found on neurons, mast cells, neural stem cells and ependymal cells lining the ventricles of the brain. It is known to cleave chondroitin sulfate, dermatan sulfate, pro-MMP-2, and N-Cadherin. Its activation of MMP-2 likely contributes to neurite outgrowth, while its cleavage of N-Cadherin in-trans between neural stem cells and ependymal initiates stem cell expansion and proliferation. Over amino acids 125-296, mouse MMP-24 shares 100% and 99% aa sequence identity with rat and human MMP-24, respectively.

Long Name

Matrix Metalloproteinase 24/Membrane Type 5 MMP

Alternate Names

MMP24, MT5-MMP

Entrez Gene IDs

10893 (Human); 17391 (Mouse)

Gene Symbol

MMP24

UniProt

Q9R0S2

Additional MMP-24/MT5-MMP Products

Product Documents for Human/Mouse MMP‑24/MT5‑MMP PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse MMP‑24/MT5‑MMP PE‑conjugated Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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