Human/Mouse Neuropilin-2 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB22151A
Detection of Neuropilin‑2 in HUVEC Human Cells by Flow Cytometry.
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Product Details
Citations (2)
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Human/Mouse Neuropilin-2 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human Neuropilin-2 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat (rr) Neuropilin-1 or rrNeuropilin-2 is observed. Detects mouse Neuropilin-2 in flow cytometry.
Source
Monoclonal Mouse IgG2A Clone # 257103
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neuropilin‑2
Gln23-Tyr855
Accession # NP_003863
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Neuropilin‑2 antibody in HUVEC Human Cells antibody by Flow Cytometry. View Larger

Detection of Neuropilin‑2 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human/Mouse Neuropilin-2 APC-conjugated Monoclonal Antibody (Catalog # FAB22151A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Neuropilin‑2 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuropilin‑2 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse Anti-Human/Mouse Neuropilin-2 APC-conjugated Monoclonal Antibody (Catalog # FAB22151A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Neuropilin-2

Neuropilin-2 (Npn-2) is a 120 kDa, type I transmembrane (TM) glycoprotein that is related to the semaphorin receptor Neuropilin-1 (1). Npn-2 is a complex molecule with multiple splice forms. Five transmembrane forms are known, and one 62 kDa soluble form has been identified (2). Based on the originally reported precursor size of 909 amino acids (aa), the “standard” precursor in human will have a 20 aa signal sequence, an 842 aa extracellular region, a 25 aa TM segment, and a 42 aa cytoplasmic tail (1). The extracellular region contains two N-terminal CUB (C1r/Ugef/BMP-1) domains, two jellyroll-shaped coagulation factor V type C domains, and a juxtamembrane MAM (Meprin/A-5 protein/tyrosine phosphatase Mu) domain (1, 3). The CUB and factor V domain are involved in VEGF and semaphorin binding. The MAM domain appears necessary for signaling through plexin-1 (4). The five transmembrane isoforms all share the same CUB, factor V and MAM domains. Splicing begins at aa 809, seven amino acids after the end of the MAM domain, and it involves the end of the extracellular region, the TM segment, and the cytoplasmic domain (a total of 101 aa). Two of the four variants show a complete replacement of these 101 aa with a totally unrelated stretch of approximately 90 aa. This creates a new TM and cytoplasmic tail. These forms are called “Npn-2b” forms. Two other isoforms (plus the standard 909 aa form) retain the 101 aa stretch, and add either 17 or 22 aa to the end of the extracellular region. These forms are called “Npn-2a” forms. The isoform offered by R&D Systems is the “a” form with the 17 aa addition. This isoform shows 94% aa identity to the equivalent regions in mouse and rat Npn-2. The soluble form of Npn-2 is 555 aa in precursor length, and contains the two CUB domains plus the first 1½ factor V type C domains (1). Npn-2 binds Sema3B through F, and VEGF isoforms 165, 145, PlGF-2 and VEGF-C (5). It is known to form homodimers and heterodimers with Npn-1, and it forms receptor complexes with plexin-1 and VEGFR1 (4, 5). Npn-2 is found on a variety of cell types including neurons (motor, autonomic, sensory), vascular endothelial cells, Schwann cells and pancreatic acinar cells.

References
  1. Chen, H. et al. (1997) Neuron 19:547.
  2. Rossignol, M. et al. (2000) Genomics 70:211.
  3. He, Z. and M. Tessier-lavigne (1997) Cell 90:739.
  4. Nakamura, F. and Y. Goshima (2002) Adv. Exp. Med. Biol. 515:55.
  5. Neufeld, G. et al. (2002) Adv. Exp. Med. Biol. 515:81.
Entrez Gene IDs
8828 (Human); 18187 (Mouse); 81527 (Rat)
Alternate Names
MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

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Citations for Human/Mouse Neuropilin-2 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. VEGF-C mediates tumor growth and metastasis through promoting EMT-epithelial breast cancer cell crosstalk
    Authors: D Kong, H Zhou, D Neelakanta, CJ Hughes, JY Hsu, RR Srinivasan, MT Lewis, HL Ford
    Oncogene, 2020;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Semaphorin3A-neuropilin1 signalling is involved in the generation of cortical interneurons
    Authors: John G Parnavelas
    Brain Struct Funct, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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