|Detection of Human/Mouse NRARP by Western Blot. Western blot shows lysates of human placenta, liver, and kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NRARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4814) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NRARP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
NRARP (Notch-regulated ankyrin repeat protein) is a 13 kDa (predicted) protein that is induced by Notch activation. In SDS-PAGE, this protein migrates at 25‑30 kDa. It is expressed in multiple cell types such as fibroblasts and thymocyte precursors. NRARP is presumed to block Notch1-mediated conversion of the CSL transcription factor from a transcriptional suppressor to activator. It is also a positive regulator of the Wnt pathway by stabilizing LEF1. Human NRARP is 114 amino acids (aa) in length. It contains two C-terminal ankyrin-like repeats (aa 50‑82 and 83‑114) that may be involved in protein-protein interactions. Human and mouse NRARP are identical in amino acid sequence.
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