Human/Mouse NRARP Antibody

R&D Systems | Catalog # AF4814

R&D Systems
Discontinued Product
AF4814 has been discontinued. View all NRARP products.

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NRARP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NRARP
Ser2-Arg114
Accession # Q7Z6K4

Specificity

Detects human and mouse NRARP in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse NRARP Antibody

Detection of Human/Mouse NRARP antibody by Western Blot.

Detection of Human/Mouse NRARP by Western Blot.

Western blot shows lysates of human placenta, liver, and kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse NRARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4814) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for NRARP at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse NRARP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human placenta, liver and kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NRARP

NRARP (Notch-regulated ankyrin repeat protein) is a 13 kDa (predicted) protein that is induced by Notch activation. In SDS-PAGE, this protein migrates at 25‑30 kDa. It is expressed in multiple cell types such as fibroblasts and thymocyte precursors. NRARP is presumed to block Notch1-mediated conversion of the CSL transcription factor from a transcriptional suppressor to activator. It is also a positive regulator of the Wnt pathway by stabilizing LEF1. Human NRARP is 114 amino acids (aa) in length. It contains two C-terminal ankyrin-like repeats (aa 50‑82 and 83‑114) that may be involved in protein-protein interactions. Human and mouse NRARP are identical in amino acid sequence.

Long Name

Notch-Regulated Ankyrin Repeat Protein

Alternate Names

MGC61598, NOTCH-regulated ankyrin repeat protein, notch-regulated ankyrin repeat-containing protein

Entrez Gene IDs

441478 (Human); 67122 (Mouse); 499745 (Rat)

Gene Symbol

NRARP

UniProt

Q7Z6K4

Additional NRARP Products

Product Documents for Human/Mouse NRARP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse NRARP Antibody

For research use only

Related Research Areas

Notch Pathway

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail