Goat IgG Horseradish Peroxidase-conjugated Antibody

Name: Goat IgG Horseradish Peroxidase-conjugated Antibody
Brand: R&D Systems

(7 citations)

Datasheet / CoA / SDS

Western Blot

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Product Details

Citations (7)

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Species Reactivity

Goat
Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.
Source

Polyclonal Chicken IgY
Purification

Antigen Affinity-purified from egg yolks
Immunogen

Goat IgG
Formulation

Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.
Label

HRP

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

Use at a 1:1000 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Goat IgG Primary Antibody byWestern Blot. Western blot shows detection of Goat Anti-Human/Mouse/RatCarboxylesterase 1/CES1 Polyclonal Antibody (Catalog # AF4920)using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody(Catalog # HAF019). This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
- 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name:

Immunoglobulin G

Alternate Names:

IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species	Applications	Sample Type
Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels Authors: E Casta¤o, L Caviedes, S Hirsch, M Llanos, G I¤iguez, AM Ronco PLoS ONE, 2017;12(1):e0170389. Species: Human Sample Type: Tissue Homogenates Application: WB Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma J Immunol Res, 2017;2017(0):6468097. Species: Human Sample Type: Plasma Application: WB Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang Oncol Lett, 2017;14(6):8183-8189. Species: Human Sample Type: Whole Tissue Application: IHC-P Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice. Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K J Immunol, 2015;195(3):1233-41. Species: Mouse Sample Type: Cell Culture Supernates Application: WB Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma. Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M Biomark Insights, 2012;7(0):87-104. Species: Mouse Sample Type: BALF Application: WB BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation. Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D PLoS ONE, 2010;5(9):e12995. Species: Mouse Sample Type: Tissue Homogenates Application: WB Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors. Authors: Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH Mol. Ther., 2009;17(10):1683-91. Species: Human Sample Type: Tissue Homogenates Application: WB

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Secondary Antibodies

Description Application Cat# Citations Images

Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20

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Goat IgG Horseradish Peroxidase-conjugated Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

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