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Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019 17 Citations Datasheet / COA / SDS
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HAF019

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Detection of Goat IgG Primary Antibody by Western Blot.
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Citations (17)
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Goat IgG Horseradish Peroxidase-conjugated Antibody Summary

Species Reactivity
Goat
Specificity
Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.
Source
Polyclonal Chicken IgY
Purification
Antigen Affinity-purified from egg yolks
Immunogen
Goat IgG
Formulation
Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Goat IgG Primary Antibody by Western Blot. View Larger

Detection of Goat IgG Primary Antibody by Western Blot. Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (Catalog # AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Goat IgG Horseradish Peroxidase-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Oct4 differentially regulates chromatin opening and enhancer transcription in pluripotent stem cells
    Authors: L Xiong, EA Tolen, J Choi, S Velychko, L Caizzi, T Velychko, K Adachi, CM MacCarthy, M Lidschreib, P Cramer, HR Schöler
    Elife, 2022;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Hepatocyte Nuclear Factor 1alpha Proinflammatory Effect Linked to the Overexpression of Liver Nuclear Factor-kappaB in Experimental Model of Chronic Kidney Disease
    Authors: E Sucajtys-S, A Debska-Sli, B Rutkowski, M Szolkiewic, J Swierczyns, RT Smolenski
    International Journal of Molecular Sciences, 2022;23(16):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS
    Authors: YG Gete, LW Koblan, X Mao, M Trappio, B Mahadik, JP Fisher, DR Liu, K Cao
    Aging Cell, 2021;0(0):e13388.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Donor cell memory confers a metastable state of directly converted cells
    Authors: KP Kim, C Li, D Bunina, HW Jeong, J Ghelman, J Yoon, B Shin, H Park, DW Han, JB Zaugg, J Kim, T Kuhlmann, RH Adams, KM Noh, SA Goldman, HR Schöler
    Cell Stem Cell, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs
    Authors: S Zalba, AM Contreras-, E Martisova, R Debets, C Smerdou, MJ Garrido
    Pharmaceutics, 2020;12(6):.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Detection
  6. Sialylation of FGFR1 by ST6Gal?I overexpression contributes to ovarian cancer cell migration and chemoresistance
    Authors: L Ou, X He, N Liu, Y Song, J Li, L Gao, X Huang, Z Deng, X Wang, S Lin
    Mol Med Rep, 2020;21(3):1449-1460.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Reprogramming competence of OCT factors is determined by transactivation domains
    Authors: KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler
    Science Advances, 2020;6(36):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats
    Authors: Y Wang, Y Mi, J Tian, X Qiao, X Su, J Kang, Z Wu, G Wang, X Zhou, Y Zhou, R Li
    Drug Des Devel Ther, 2020;14(0):4825-4834.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains
    Authors: F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert
    Arthritis & rheumatology (Hoboken, N.J.), 2018;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Western Blot
  10. Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1
    Authors: X Miao, MF Rahman, L Jiang, Y Min, S Tan, H Xie, L Lee, M Wang, RE Malmström, WO Lui, N Li
    J. Thromb. Haemost., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
    Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
    J Immunol Res, 2017;2017(0):6468097.
    Species: Human
    Sample Types: Plasma
    Applications: Western Blot
  12. Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma
    Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang
    Oncol Lett, 2017;14(6):8183-8189.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels
    Authors: E Casta¤o, L Caviedes, S Hirsch, M Llanos, G I¤iguez, AM Ronco
    PLoS ONE, 2017;12(1):e0170389.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice.
    Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K
    J Immunol, 2015;195(3):1233-41.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  15. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  16. BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
    Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
    PLoS ONE, 2010;5(9):e12995.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  17. Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors.
    Authors: Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH
    Mol. Ther., 2009;17(10):1683-91.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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