Goat IgG Horseradish Peroxidase-conjugated Antibody

HAF019 12 Citations Datasheet / COA / SDS

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HAF019

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Detection of Goat IgG Primary Antibody by Western Blot.

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Citations (12)

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Goat IgG Horseradish Peroxidase-conjugated Antibody Summary

Species Reactivity

Goat

Specificity

Detects goat IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross‑reactivity with mouse IgG, rabbit IgG and human IgG is observed.

Source

Polyclonal Chicken IgY

Purification

Antigen Affinity-purified from egg yolks

Immunogen

Goat IgG

Formulation

Supplied as a 0.2 μm filtered solution in Sodium Phosphate and Proclin 300.

Label

HRP(Excitation= nm, Emission= nm)

Applications

Recommended Concentration

Sample

Western Blot

Use at a 1:1000 dilution.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot

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Detection of Goat IgG Primary Antibody by Western Blot. Western blot shows detection of Goat Anti-Human/Mouse/Rat Carboxylesterase 1/CES1 Polyclonal Antibody (Catalog # AF4920) using a 1:1000 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

IgG

Product Datasheets

Product Datasheet

COA

SDS

Citations for Goat IgG Horseradish Peroxidase-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats
Authors: Y Wang, Y Mi, J Tian, X Qiao, X Su, J Kang, Z Wu, G Wang, X Zhou, Y Zhou, R Li
Drug Des Devel Ther, 2020;14(0):4825-4834.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC
Quantification of Pharmacokinetic Profiles of PD-1/PD-L1 Antibodies by Validated ELISAs
Authors: S Zalba, AM Contreras-, E Martisova, R Debets, C Smerdou, MJ Garrido
Pharmaceutics, 2020;12(6):.
Species: Mouse
Sample Types: Plasma
Applications: ELISA Detection
Reprogramming competence of OCT factors is determined by transactivation domains
Authors: KP Kim, Y Wu, J Yoon, K Adachi, G Wu, S Velychko, CM MacCarthy, B Shin, A Röpke, MJ Arauzo-Bra, M Stehling, DW Han, Y Gao, J Kim, S Gao, HR Schöler
Science Advances, 2020;6(36):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
The Role of Autophagy in the Degradation of Misfolded HLA-B27 Heavy Chains
Authors: F Navid, G Layh-Schmi, KA Sikora, A Cougnoux, RA Colbert
Arthritis & rheumatology (Hoboken, N.J.), 2018;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Western Blot
Thrombin-reduced miR-27b attenuates platelet angiogenic activities in vitro via enhancing platelet synthesis of anti-angiogenic thrombospondin-1
Authors: X Miao, MF Rahman, L Jiang, Y Min, S Tan, H Xie, L Lee, M Wang, RE Malmström, WO Lui, N Li
J. Thromb. Haemost., 2018;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels
Authors: E Casta¤o, L Caviedes, S Hirsch, M Llanos, G I¤iguez, AM Ronco
PLoS ONE, 2017;12(1):e0170389.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot
Role of CCL20/CCR6 and the ERK signaling pathway in lung adenocarcinoma
Authors: XP Zhang, ZJ Hu, AH Meng, GC Duan, QT Zhao, J Yang
Oncol Lett, 2017;14(6):8183-8189.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Sustained High Levels of Both Total and High Molecular Weight Adiponectin in Plasma during the Convalescent Phase of Haemorrhagic Fever with Renal Syndrome Are Associated with Disease Severity
Authors: K Tang, C Zhang, Y Zhang, Y Zhang, R Zhuang, B Jin, Y Ma
J Immunol Res, 2017;2017(0):6468097.
Species: Human
Sample Types: Plasma
Applications: Western Blot
Deficient NLRP3 and AIM2 Inflammasome Function in Autoimmune NZB Mice.
Authors: Sester D, Sagulenko V, Thygesen S, Cridland J, Loi Y, Cridland S, Masters S, Genske U, Hornung V, Andoniou C, Sweet M, Degli-Esposti M, Schroder K, Stacey K
J Immunol, 2015;195(3):1233-41.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot
Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
Biomark Insights, 2012;7(0):87-104.
Species: Mouse
Sample Types: BALF
Applications: Western Blot

BAMBI is expressed in endothelial cells and is regulated by lysosomal/autolysosomal degradation.
Authors: Xavier S, Gilbert V, Rastaldi MP, Krick S, Kollins D, Reddy A, Bottinger E, Cohen CD, Schlondorff D
PLoS ONE, 2010;5(9):e12995.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors.
Authors: Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH
Mol. Ther., 2009;17(10):1683-91.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot