Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 274212
View all Pax3/Pax7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax3 (isoform Pax3a)
Met1-Ser215
Accession # NP_000429

Specificity

Detects human and mouse Pax3 and Pax7 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Pax1 is observed, approximately 10% with rhPax6, and less than 2% with rhPax2, rhPax4, rhPax5, and rhPax9.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse Pax3/Pax7 /Pax7 APC‑conjugated Antibody

Detection of Pax3/Pax7 in B16-F1 cells by Flow Cytometry

B16-F1 cells were stained with Mouse Anti-Human/Mouse Pax3/Pax7 APC‑conjugated Monoclonal Antibody (Catalog # IC2457A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Pax3/Pax7 /Pax7 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: B16‑F1 mouse melanoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pax3/Pax7

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.

Long Name

Paired Box Gene 3 /Paired Box Gene 7

UniProt

NP_000429

Additional Pax3/Pax7 Products

Product Documents for Human/Mouse Pax3/Pax7 /Pax7 APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Pax3/Pax7 /Pax7 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse Pax3/Pax7 /Pax7 APC‑conjugated Antibody

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