Human/Mouse Pax3 PE-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Pax3 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 274212
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Pax3 (isoform Pax3a)
    Met1-Ser215
    Accession # NP_000429
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Pax3 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Mouse Anti-Human/Mouse Pax3 PE‑conjugated Monoclonal Antibody (Catalog # IC2457P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Pax3
Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. Human and mouse Pax3 share 98% amino acid sequence identity.
  • Long Name:
    Paired Box Gene 3 [Waardenburg Syndrome 1]
  • Entrez Gene IDs:
    5077 (Human); 18505 (Mouse)
  • Alternate Names:
    CDHS; HUP2; HUP2MGC120384; MGC120381; MGC120382; MGC120383; MGC134778; paired box 3; paired box gene 3 (Waardenburg syndrome 1); paired box homeotic gene 3; paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; Pax3; Waardenburg syndrome 1; WS1; WS3
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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