Detection of Androgen R/ NR3C4 in LNCaP Human Cell Line by Flow Cytometry.
LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 Monoclonal Antibody (Catalog # MAB5876, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Androgen R/NR3C4
ligand binding domain of human AR (aa 661-920) shares 100% aa sequence
identity with mouse and rat AR. AR (Androgen receptor) is a 99 kDa
(predicted) member of the NR3 subfamily, nuclear hormone receptor family
of proteins. Due to a high number of Gln and Pro residues, it runs
anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being
found in neurons, endothelial cells, osteoblasts, chrondrocytes,
mascrophages, adipocytes, and prostate epithelium. Human AR is 919 amino
acids (aa) in length. It contains three discrete domains: a
"modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a
Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain
(aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln
and Gly residues differing by seven or more residues among individuals.
Multiple potential splice forms exist, including an alternative start
site at Met189 and a seven aa substitution for aa 1‑538 that generates a
45 kDa isoform. AR does homodimerize, apparently with multiple
isotypes. Over aa 661‑920, human and mouse are identical in aa sequence.
Entrez Gene IDs:
AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy
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