Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC5876A
Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody in Data
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Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Androgen R/NR3C4 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 523339
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Androgen R/NR3C4
Thr660-Gln919
Accession # P10275
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Androgen R/NR3C4 in LNCaP Human Cell Line by Flow Cytometry. View Larger

Detection of Androgen R/NR3C4 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Monoclonal Antibody (Catalog # IC5876A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Androgen R/NR3C4

The AR (Androgen Receptor) is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, mascrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR homodimerizes, and may heterodimerize, with its isoforms. Over aa 660‑919, human, mouse, and rat are identical in aa sequence.

Long Name
Androgen Receptor
Entrez Gene IDs
367 (Human)
Alternate Names
AIS; Androgen R; androgen receptor; AndrogenR; AR; DHTR; DHTRTFM; Dihydrotestosterone receptorHYSP1; HUMARA; NR3C4; NR3C4KD; Nuclear receptor subfamily 3 group C member 4; SBMA; SMAX1; SMAX1SBMA; spinal and bulbar muscular atrophy

Product Datasheets

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Reviews for Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody

Average Rating: 5 (Based on 7 Reviews)

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Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 04/12/2018
Application: Flow Sample Tested: Cultured rat embryonic hippocampal neurons Species: Rat

Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 03/30/2018
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 03/14/2018
Application: Flow Sample Tested: F9 mouse teratocarcinoma stem cells Species: Rat

Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 03/08/2018
Application: Flow Sample Tested: LNCaP human prostate cancer cell line Species: Human

Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 03/07/2018
Application: Flow Sample Tested: A375 human melanoma cell line Species: Human

Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 02/14/2018
Application: Flow Sample Tested: LNCaP human prostate cancer cell line Species: Human

Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
By Anonymous on 02/08/2018
Application: Flow Sample Tested: Primary culture Species: Human