Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody Summary
Accession # P10275
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Androgen R/NR3C4 in LNCaP Human Cell Line by Flow Cytometry. LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Monoclonal Antibody (Catalog # IC5876A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Androgen R/NR3C4
The AR (Androgen Receptor) is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, mascrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR homodimerizes, and may heterodimerize, with its isoforms. Over aa 660‑919, human, mouse, and rat are identical in aa sequence.
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