Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody

R&D Systems | Catalog # IC5876A

R&D Systems
Discontinued Product
IC5876A has been discontinued. View all Androgen R/NR3C4 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 523339
View all Androgen R/NR3C4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Androgen R/NR3C4
Thr660-Gln919
Accession # P10275

Specificity

Detects human Androgen R/NR3C4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody

Detection of Androgen R/NR3C4 antibody in LNCaP Human Cell Line antibody by Flow Cytometry.

Detection of Androgen R/NR3C4 in LNCaP Human Cell Line by Flow Cytometry.

LNCaP human prostate cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Monoclonal Antibody (Catalog # IC5876A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: LNCaP human prostate cancer cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 7 reviews rated 5 using IC5876A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Androgen R/NR3C4

The AR (Androgen Receptor) is a 99 kDa (predicted) member of the NR3 subfamily, nuclear hormone receptor family of proteins. Due to a high number of Gln and Pro residues, it runs anomalously at 100‑120 kDa in SDS-PAGE. It is widely expressed, being found in neurons, endothelial cells, osteoblasts, chrondrocytes, mascrophages, adipocytes, and prostate epithelium. Human AR is 919 amino acids (aa) in length. It contains three discrete domains: a "modulating" N‑terminus (aa 1‑553) that is rich in Gln, Pro and Gly, a Zn-finger DNA-binding region (aa 554‑635), and a ligand-binding domain (aa 672‑917). AR is highly polymorphic at the N‑terminus, with total Gln and Gly residues differing by seven or more residues among individuals. Multiple potential splice forms exist, including an alternative start site at Met189 and a seven aa substitution for aa 1‑538 that generates a 45 kDa isoform. AR homodimerizes, and may heterodimerize, with its isoforms. Over aa 660‑919, human, mouse, and rat are identical in aa sequence.

Long Name

Androgen Receptor

Alternate Names

AIS, AndrogenR, AR, DHTR, NR3C4, SBMA, SMAX1

Entrez Gene IDs

367 (Human)

Gene Symbol

AR

UniProt

P10275

Additional Androgen R/NR3C4 Products

Product Documents for Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody

Customer Reviews for Human/Mouse/Rat Androgen R/NR3C4 APC‑conjugated Antibody (7)

5 out of 5
7 Customer Ratings
5 Stars
100%
4 Stars
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1 Stars
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Showing  1 - 5 of 7 reviews Showing All
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  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Cultured rat embryonic hippocampal neurons
    Species: Rat
    Verified Customer | Posted 04/12/2018
  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 4T1 mouse breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 03/30/2018
  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: F9 mouse teratocarcinoma stem cells
    Species: Rat
    Verified Customer | Posted 03/14/2018
  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: LNCaP human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 03/08/2018
  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Shub Poddar
    Application: Flow Cytometry
    Sample Tested: A375 human melanoma cell line
    Species: Human
    Verified Customer | Posted 03/07/2018
  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: LNCaP human prostate cancer cell line
    Species: Human
    Verified Customer | Posted 02/14/2018
  • Human/Mouse/Rat Androgen R/NR3C4 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Primary culture
    Species: Human
    Verified Customer | Posted 02/08/2018

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail