Human/Mouse/Rat BOK Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: BOK

BOK (Bcl-2-related ovarian killer protein; also Bcl2L9 and Mtd) is a 27 kDa member of the Bax subgroup of the Bcl-2 family of molecules. It is expressed in spermatocytes, ovarian granulosa cells, and liver cells. BOK is activated by E2F proteins, and sensitizes replicating cells to apoptosis when under stress. It is normally found in the cytosol, and upon activation, becomes incorporated into mitochondrial membranes. Human BOK is 212 amino acids (aa) in length and contains one BH4 domain (aa 32‑44), one BH3 domain (aa 66‑82), a BH1 domain (aa 112‑131) and a BH2 domain (aa 164‑178). A potential transmembrane span has been identified between aa 189‑210. BOK heterodimerizes with Mcl-1, BHRF-1, Bfl-1 and Bnip3, and is known to form heterooligomers. There are two potential splice variants. One contains an alternative start site at Met145, while another shows a five aa substitution for aa 117‑212. Over aa 15‑123, human BOK shares 94% aa identity with mouse BOK.

Product Datasheets

Related Research Areas

• Bcl-2 Proteins and Regulators