Caveolin‑2 in HeLa Human Cell Line. Caveolin‑2 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Caveolin‑2 Alexa Fluor® 488‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5788G) at 1:10 dilution overnight at 4° C and counterstained with DAPI (blue). Specific staining was localized to caveolae. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Caveolin-2 is a cell membrane-associated member of the caveolin family of proteins. Although its predicted MW is 17 kDa, it runs anomalously at 21‑22 kDa on SDS‑PAGE. Caveolin-2 is not ubiquitous, but it is found in divergent cell types such as smooth muscle, endothelial, and skeletal muscle cells, plus fibroblasts and adipocytes. When expressed alone, Caveolin-2 forms monomers and homodimers; when expressed with Caveolin-1, it exists as part of a 150 kDa, Caveolin-1 associated oligomeric complex. Human Caveolin-2 is 162 amino acids (aa) in length. It contains a central membrane-embedded sequence (aa 87‑107) flanked by two cytoplasmic domains (aa 1‑86 and 108‑162). Caveolin-2 has been found phosphorylated at tyrosines 19 and 27, and at serines 20, 23 and 36.
Entrez Gene IDs:
858 (Human); 12390 (Mouse); 363425 (Rat)
CAV; CAV2; caveolae protein, 20-kD; caveolin 2 isoform a and b; caveolin 2; Caveolin2; caveolin-2; MGC12294
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.