Detection of GABAA R alpha 4 N-Terminus by Western Blot. Western blot of rat brain (hippocampal) lysates using approximately 5 - 7 μg of tissue per lane. The blot was incubated overnight at 2 - 8° C with anti-GABAA R, alpha 4 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~64 kDa alpha 4 subunit of the GABAA R.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R alpha 4
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 4 isoform is a 67 kDa, 517 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 14 - 221). The alpha 4 subunit is unusual in that it does not seem to preferentially form channel complexes with the gamma subunit. In hippocampal neurons, alpha 4 subunits apparently form GABA receptors that do not cluster, but exist in diffuse net-works in nonsynaptic membranes.
Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Wisden, W. et al. (1991) FEBS Lett. 289:227.
Bencsits, E. et al. (1999) J. Biol. Chem. 274:19613.
Mangan, P.S. et al. (2005) Mol. Pharmacol. 67:775.
gamma-Aminobutyric Acid Type A Receptor, alpha-4 Polypeptide
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