Human/Mouse/Rat Glyoxalase II Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # AF5944AFP488

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Goat IgG
View all Glyoxalase II Primary Antibodies »

Product Specifications

Specificity

Detects human, mouse, and rat Glyoxalase II in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Glyoxalase I is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Glyoxalase II

Glyoxalase II (GLO2; also hydroxyacylglutathione hydrolase 2 and Glx II) is a monomeric, cytosolic 29 kDa member of the Glyoxylase II family, metallo-beta -lactamase superfamily of enzymes. It is expressed in liver and kidney, and converts GLO1-generated lactoylglutathione into lactate and GSH. It is up‑regulated by p63 and p73, and thus serves as a p53-associated survival factor. Human GLO2 is 260 amino acids (aa) in length. It binds two atoms of Zn and contains one substrate binding region (aa 143‑145 plus 249‑252). The 29 kDa form represents 80‑90% of cellular GLO2. There is also a 32‑33 kDa, 308 aa mitochondrial isoform that arises via the use of an alternative start site. Its function is unclear, given that mitochondria do not contain GLO1. There is an additional potential isoform that shows an insertion of nine aa after Thr167. Human GLO2 shares approximately 90% aa identity with mouse GLO2.

Alternate Names

GLX2, HAGH

Entrez Gene IDs

3029 (Human); 14651 (Mouse); 24439 (Rat)

Gene Symbol

HAGH

UniProt

Q16775 (Human)

Additional Glyoxalase II Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Redox Enzymes

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