Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

Catalog # Availability Size / Price Qty
PPS054
Detection of GRIN2A/NMDAR2A by Western Blot.
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Human/Mouse/Rat GRIN2A/NMDAR2A Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Human, mouse, and rat ~180 kDa NMDA NR2A
Source
Polyclonal Rabbit Serum
Purification
Antigen Affinity-purified
Immunogen
NMDA R, NR2A Subunit
Formulation
50 μL of unpurified rabbit serum.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of GRIN2A/NMDAR2A by Western Blot. View Larger

Detection of GRIN2A/NMDAR2A by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2A subunit of the NMDA Receptor.

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2A/NMDAR2A

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitute the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

References
  1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
  2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
  3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
  4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A
Entrez Gene IDs
2903 (Human); 14811 (Mouse); 24409 (Rat)
Alternate Names
EPND; FESD; GluN2A; GRIN2A; LKS; NMDA R, NR2A Subunit; NMDAR2A; NR2A

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