Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Human, mouse, and rat ~180 kDa NMDA NR2A
  • Source
    Polyclonal Rabbit Serum
  • Purification
    Antigen Affinity-purified
  • Immunogen
    NMDA R, NR2A Subunit
  • Formulation
    50 μL of unpurified rabbit serum.
  • Label
  • Western Blot
    1:1000 dilution
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GRIN2A/NMDAR2A by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2A subunit of the NMDA Receptor.
Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GRIN2A/NMDAR2A
NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitute the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.
  • References:
    1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
    2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
    3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
    4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
  • Long Name:
    Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A
  • Entrez Gene IDs:
    2903 (Human); 14811 (Mouse); 24409 (Rat)
  • Alternate Names:
    EPND; FESD; GluN2A; LKS; NMDA R, NR2A Subunit; NMDAR2A; NR2A
Related Research Areas
Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

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