Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

R&D Systems | Catalog # PPS054

R&D Systems
Discontinued Product
PPS054 has been discontinued. View all GRIN2A/NMDAR2A products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum
View all GRIN2A/NMDAR2A Primary Antibodies »

Product Specifications

Immunogen

NMDA R, NR2A Subunit

Specificity

Human, mouse, and rat ~180 kDa NMDA NR2A

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Scientific Data Images for Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

Detection of GRIN2A/NMDAR2A antibody by Western Blot.

Detection of GRIN2A/NMDAR2A by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2A subunit of the NMDA Receptor.

Applications for Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat hippocampal lysate

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50 μL of unpurified rabbit serum.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2A/NMDAR2A

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitute the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

References

  1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
  2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
  3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
  4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A

Alternate Names

EPND, FESD, GluN2A, LKS, NMDA R, NR2A Subunit, NMDAR2A, NR2A

Entrez Gene IDs

2903 (Human); 14811 (Mouse); 24409 (Rat)

Gene Symbol

GRIN2A

Additional GRIN2A/NMDAR2A Products

Product Documents for Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

For research use only

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