Human/Mouse/Rat Kirrel3/NEPH2 Biotinylated Antibody

Catalog #: BAF4910
2 Citations Datasheet / COA / SDS

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BAF4910

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Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Kirrel3/NEPH2 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects Kirrel3/NEPH2 in Western blots. In Western blots, less than 2% cross‑reactivity with recombinant human (rh) Kirrel1 and rhKirrel2 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Kirrel3/NEPH2
Tyr33-Ala535
Accession # Q8IZU9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kirrel3/NEPH2

Kirrel3 (Kin of IRRE-like protein 3 precursor; also NEPH2) is a 95-120 kDa member of a small family of podocin binding molecules. Kirrel3 is found on podocytes and olfactory sensory neurons and likely plays a role in cellular adhesion. Kirrel3 is 778 amino acids in length. It is a type I transmembrane (TM) glycoprotein that contains a 514 aa extracellular region (aa 22-535) with five C2 type Ig like domains (aa 48-515) plus a 222 aa cytoplasmic tail. Kirrel3 binds itself as well as nephrin, podocin and ZO-1. Kirrel3 is shed by MMPs, generating a soluble urinary fragment that is 25 kDa smaller than the TM form. Two variant isoforms exist. One has an alternate start site at Met34, while the second shows a 35 aa substitution for aa 566-778. Over aa 1-535, human Kirrel3 is 98% aa identical to mouse Kirrel3.

Long Name

Kin of Irregular Chiasm-like 3

Entrez Gene IDs

84623 (Human); 67703 (Mouse); 315546 (Rat)

Alternate Names

KIAA1867MGC126824; kin of IRRE like 3 (Drosophila); Kin of irregular chiasm-like protein 3; kin of IRRE-like protein 3; KIRRE; Kirrel3; KIRREPRO4502; mKIRRE; MRD4; NEPH2; NEPH2MGC126850; nephrin-like 2; Nephrin-like protein 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Rat Kirrel3/NEPH2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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PGC1alpha is required for the renoprotective effect of lncRNA Tug1 inï¿½vivo and links Tug1 with urea cycle metabolites
Authors: L Li, J Long, K Mise, DL Galvan, PA Overbeek, L Tan, SV Kumar, WK Chan, PL Lorenzi, BH Chang, FR Danesh
Cell Reports, 2021;36(6):109510.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Role for carbohydrate response element-binding protein (ChREBP) in high glucose-mediated repression of long noncoding RNA Tug1
Authors: J Long, DL Galvan, K Mise, YS Kanwar, L Li, N Poungvarin, PA Overbeek, BH Chang, FR Danesh
J. Biol. Chem., 2020;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Isolation