Human/Mouse/Rat MKK6 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF16041S Datasheet / COA / SDS

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AF16041S-100UG

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All MKK6 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat MKK6 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat MKK6.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MKK6
Ser2-Asp334
Accession # P52564

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Knockout Validated

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MKK6

Mitogen-activated protein kinase kinase 6 (MKK6), also known as MEK6, and the closely related MKK3 are activated by pro-inflammatory cytokines and environmental stresses (1, 2). Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr (3). Activation of MKK6 occurs through phosphorylation at Ser207 and Thr211 (4) by several upstream MAPK kinase kinases, including ASK1 and TAK1 (5, 6). Purification of E. coli-expressed wild-type active MKK6 capable of p38 alpha phosphorylation has been previously noted (7).

Long Name

Mitogen-activated Protein Kinase Kinase 6

Entrez Gene IDs

5608 (Human); 26399 (Mouse); 114495 (Rat)

Alternate Names

MAP kinase kinase 6; MAP2K6; MAPK/ERK kinase 6; MAPKK6dual specificity mitogen-activated protein kinase kinase 6; MEK6; MEK6MAPKK 6; mitogen-activated protein kinase kinase 6; MKK6; MKK6MEK 6; PRKMK6; PRKMK6protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6); SAPKK3; SAPKK3EC 2.7.12.2