Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Sheep IgG
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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Nicalin
Met1-Ala522, predicted
Accession # Q969V3

Specificity

Detects human, mouse, and rat Nicalin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human/Mouse/Rat Nicalin Alexa Fluor® 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nicalin

Nicalin (NICAstrin-Like proteIN) is a 60-62 kDa member of the nicastrin family of proteins. It is an ER-embedded transmembrane protein that is found in skeletal and cardiac muscle, hepatocytes and neurons. Nicalin interacts with 22 kDa TMEM147 and 130 kDa NOMO1 to form a 200-220 kDa complex that antagonizes Nodal signaling. Mature human nicalin is a 521 amino acid (aa) type I transmembrane glycoprotein. It contains a 480 aa luminal/extracellular region (aa 43-522) plus a 20 aa cytoplasmic domain (aa 544-563). The luminal region possesses one likely inactive M28 Zn-peptidase-like domain (aa 130-429). Four potential isoform variants are reported. One shows an alternative start site at Met75, a second contains a seven aa insertion after Gln543, a third contains a 102 aa substitution for aa 162-563, and a fourth shows a deletion of Gln446. Over aa 1-522, human nicalin shares 95% aa sequence identity with mouse nicalin.

Alternate Names

Ncl-1, NCLN, NET59

Entrez Gene IDs

56926 (Human); 103425 (Mouse); 314648 (Rat)

Gene Symbol

NCLN

UniProt

Additional Nicalin Products

Product Documents for Human/Mouse/Rat Nicalin Alexa Fluor® 405‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Nicalin Alexa Fluor® 405‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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