Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat PP2A when phosphorylated at Y307. The antigen is identical to the PP4 Y305 phosphorylation site.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human PP2A Y307 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-PP2A (Y307), Catalytic Subunit by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3989), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PP2A (Y307), Catalytic Subunit at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2A

Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.

  • Entrez Gene IDs:
    5515 (Human); 51792 (Mouse)
  • Alternate Names:
    EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.
    Authors: Wei Q, Jiang H, Xiao Z
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(17):7004-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit IgG Antibody

WB AF008 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Associated Pathways

Feedback

Customer Information