Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody

Catalog # Availability Size / Price Qty
AF3989
AF3989-SP
Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody in Data
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Product Details
Citations (6)
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Reviews (6)

Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse, and rat PP2A when phosphorylated at Y307. The antigen is identical to the PP4 Y305 phosphorylation site.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human PP2A Y307 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-PP2A (Y307), Catalytic Subunit antibody by Western Blot. View Larger

Detection of Human Phospho-PP2A (Y307), Catalytic Subunit by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3989), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PP2A (Y307), Catalytic Subunit at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP2A

Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.

Entrez Gene IDs
5515 (Human); 51792 (Mouse)
Alternate Names
EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Product Datasheets

Citations for Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation
    Authors: IE Frohner, I Mudrak, S Schüchner, D Anrather, M Hartl, JM Sontag, E Sontag, BE Wadzinski, T Preglej, W Ellmeier, E Ogris
    Cell Rep, 2020;30(9):3171-3182.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac
    Authors: S Mazhar, D Leonard, A Sosa, D Schlatzer, D Thomas, G Narla
    Cell Rep, 2020;30(9):3164-3170.e3.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Overcoming Tyrosine Kinase Inhibitor Resistance in Transformed Cell Harboring SEPT9-ABL1 Chimeric Fusion Protein
    Authors: H Kawai, H Matsushita, R Suzuki, Y Kitamura, Y Ogawa, H Kawada, K Ando
    Neoplasia, 2019;21(8):788-801.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  4. The Prenylflavonoid Xanthohumol Reduces Alzheimer-Like Changes and Modulates Multiple Pathogenic Molecular Pathways in the Neuro2a/APPswe Cell Model of AD
    Authors: X Huang, J Wang, X Chen, P Liu, S Wang, F Song, Z Zhang, F Zhu, X Huang, J Liu, G Song, PS Spencer, X Yang
    Front Pharmacol, 2018;9(0):199.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  5. The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
    Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
    Oxid Med Cell Longev, 2018;2018(0):2025914.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.
    Authors: Wei Q, Jiang H, Xiao Z
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(17):7004-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody

Average Rating: 4 (Based on 6 Reviews)

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Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
By Anonymous on 02/06/2021
Application: WB Sample Tested: Adult brain Species: Mouse

The extracted proteins were separated by SDS gel electrophoresis and transferred to nitrocellulose membranes. After incubation with the primary antibody overnight, the blots were washed in TBST and incubated at room temperature with anti-rabbit secondary antibody (1:10000 dilution).


Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
By Anonymous on 11/06/2020
Application: WB Sample Tested: Liver cells Species: Human

Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
By Anonymous on 06/10/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human

Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
By Anonymous on 11/02/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human