Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Human Phospho-PP2A (Y307), Catalytic Subunit by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line untreated (-) or treated (+) with hydroxyurea. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3989), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PP2A (Y307), Catalytic Subunit at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2A
Protein Phosphatase 2A (PP2A) dephosphorylates serine and threonine residues in proteins. This ubiquitously expressed enzyme plays a critical role in modulating cell survival, growth factor responses, and neurotransmission. Phosphorylation near the C-terminus at Y307 of the catalytic subunit decreases the phosphatase activity of PP2A and reduces its ability to bind to proteins such as CD28 and the glutamate receptor GluR5. Phosphorylation of PP2A at Y307 is highest during M-phase and lowest during S-phase of the cell cycle. Transient PP2A phosphorylation at this site is also observed in growth factor-stimulated cells.
Product Datasheets
Citations for Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
Dl-3-n-Butylphthalide Reduces Cognitive Deficits and Alleviates Neuropathology in P301S Tau Transgenic Mice
Authors: Y Chang, Y Yao, R Ma, Z Wang, J Hu, Y Wu, X Jiang, L Li, G Li
Frontiers in Neuroscience, 2021;15(0):620176.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
PP2AC Phospho-Tyr307 Antibodies Are Not Specific for this Modification but Are Sensitive to Other PP2AC Modifications Including Leu309 Methylation
Authors: IE Frohner, I Mudrak, S Schüchner, D Anrather, M Hartl, JM Sontag, E Sontag, BE Wadzinski, T Preglej, W Ellmeier, E Ogris
Cell Rep, 2020;30(9):3171-3182.e6.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Challenges and Reinterpretation of Antibody-Based Research on Phosphorylation of Tyr307 on PP2Ac
Authors: S Mazhar, D Leonard, A Sosa, D Schlatzer, D Thomas, G Narla
Cell Rep, 2020;30(9):3164-3170.e3.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Overcoming Tyrosine Kinase Inhibitor Resistance in Transformed Cell Harboring SEPT9-ABL1 Chimeric Fusion Protein
Authors: H Kawai, H Matsushita, R Suzuki, Y Kitamura, Y Ogawa, H Kawada, K Ando
Neoplasia, 2019;21(8):788-801.
Species: Human
Sample Types: Protein
Applications: Western Blot -
The Prenylflavonoid Xanthohumol Reduces Alzheimer-Like Changes and Modulates Multiple Pathogenic Molecular Pathways in the Neuro2a/APPswe Cell Model of AD
Authors: X Huang, J Wang, X Chen, P Liu, S Wang, F Song, Z Zhang, F Zhu, X Huang, J Liu, G Song, PS Spencer, X Yang
Front Pharmacol, 2018;9(0):199.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
The Isoquinoline Alkaloid Dauricine Targets Multiple Molecular Pathways to Ameliorate Alzheimer-Like Pathological Changes In Vitro
Authors: P Liu, X Chen, H Zhou, L Wang, Z Zhang, X Ren, F Zhu, Y Guo, X Huang, J Liu, PS Spencer, X Yang
Oxid Med Cell Longev, 2018;2018(0):2025914.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sulfiredoxin-Peroxiredoxin IV axis promotes human lung cancer progression through modulation of specific phosphokinase signaling.
Authors: Wei Q, Jiang H, Xiao Z
Proc. Natl. Acad. Sci. U.S.A., 2011;108(17):7004-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsIsotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody
Average Rating: 4 (Based on 6 Reviews)
Have you used Human/Mouse/Rat Phospho-PP2A (Y307), Catalytic Subunit Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
The extracted proteins were separated by SDS gel electrophoresis and transferred to nitrocellulose membranes. After incubation with the primary antibody overnight, the blots were washed in TBST and incubated at room temperature with anti-rabbit secondary antibody (1:10000 dilution).
