Human/Mouse/Rat PTEN Alexa Fluor® 700-conjugated Antibody Summary
Accession # P60484
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of PTEN in Human PBMC lymphocytes by Flow Cytometry. Human peripheral blood mononuclear cell lymphocytes were stained with Mouse Anti-Human/Mouse/Rat PTEN Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC847N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCxxGxxRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3,4,5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3,4,5)-triphosphate which is specifically produced from phosphatidylinositol (4,5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).
- Maehama, T. and J. Dixon (1998) J. Biol. Chem. 273:13375.
- Das, S. et al. (2003) Proc. Natl. Acad. Sci. USA 100:7491.
- Myers, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13513.
- Waite, K. and C. Eng (2002) Am. J. Hum. Genet. 70:829.
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