Detection of Human and Mouse TAFA5/FAM19A5 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line, T98G human glioblastoma cell line, Neuro‑2A mouse neuroblastoma cell line, and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/ FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TAFA5/FAM19A5 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of TAFA5/FAM19A5 in IMR‑32 Human Cell Line by Flow Cytometry. IMR‑32 human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat TAFA5/ FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
TAFA5 (also FAM19A5) is a 14 kDa type I transmembrane protein and member of the FAM19/TAFA family of chemokine-like proteins (1). Human TAFA5 is 132 amino acids (aa) in length. It contains a 15 aa extracellular domain, a 23 aa transmembrane sequence, and a 95 aa cytoplasmic region. Alternate splicing produces two additional isoforms. Isoform 2, a secreted form, has a 31 aa substitution for residues 1‑38 in isoform 1. Isoform 3 has an eight aa substitution for residues 1‑87 in isoform1. Human TAFA5 is 100% aa identical to mouse TAFA5 (1). Within the TAFA family, TAFA5 is the most distinct member, while TAFAs 2, 3, and 4 are the most closely related members (1). Real-time PCR analysis indicates that TAFA5 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the basal ganglia and cerebellum (1). The biological functions of TAFA family members are not yet known, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1‑2). Finally, TAFAs may control axonal sprouting following brain injury (1).
Tang, Y.T. et al. (2004) Genomics 83:727.
Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.
Family with Sequence Similarity 19, Member A5
Entrez Gene IDs:
25817 (Human); 106014 (Mouse); 500915 (Rat)
Chemokine-like protein TAFA-5; FAM19A5; family with sequence similarity 19 (chemokine (C-C motif)-like), member A5; QLLK5208; TAFA protein 5; TAFA5; TAFA-5; UNQ5208
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