Human/Mouse/Rat TAFA5/FAM19A5 Antibody

R&D Systems | Catalog # AF5148

R&D Systems
Discontinued Product
AF5148 has been discontinued. View all TAFA5/FAM19A5 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TAFA5/FAM19A5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TAFA5/FAM19A5
Gln26-Ser125
Accession # NP_056196

Specificity

Detects human and mouse TAFA5/FAM19A5 in Western blots. 

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat TAFA5/FAM19A5 Antibody

Detection of Human/Mouse/Rat TAFA5/FAM19A5 by Western Blot.

Western blot shows lysates of IMR‑32 human neuroblastoma cell line and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat TAFA5/FAM19A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TAFA5/FAM19A5 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 10.

Applications for Human/Mouse/Rat TAFA5/FAM19A5 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: IMR‑32 human neuroblastoma cell line, bEnd.3 mouse endothelioma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.  For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAFA5/FAM19A5

TAFA5 (also FAM19A5) is a 14 kDa type I transmembrane protein and member of the FAM19/TAFA family of chemokine-like proteins (1). Human TAFA5 is 132 amino acids (aa) in length. It contains a 15 aa extracellular domain, a 23 aa transmembrane sequence, and a 95 aa cytoplasmic region. Alternate splicing produces two additional isoforms. Isoform 2, a secreted form, has a 31 aa substitution for residues 1‑38 in isoform 1. Isoform 3 has an eight aa substitution for residues 1‑87 in isoform1. Human TAFA5 is 100% aa identical to mouse TAFA5 (1). Within the TAFA family, TAFA5 is the most distinct member, while TAFAs 2, 3, and 4 are the most closely related members (1). Real-time PCR analysis indicates that TAFA5 mRNA expression is restricted to the central nervous system (CNS), with the highest level in the basal ganglia and cerebellum (1). The biological functions of TAFA family members are not yet known, but there are a few tentative hypotheses. First, TAFAs may modulate immune responses in the CNS by functioning as brain-specific chemokines, and may act with other chemokines to optimize the recruitment and activity of immune cells in the CNS (1). Second, TAFAs may represent a novel class of neurokines that act as regulators of immune nervous cells (1‑2). Finally, TAFAs may control axonal sprouting following brain injury (1).

References

  1. Tang, Y.T. et al. (2004) Genomics 83:727.
  2. Benveniste, E. (1998) Cytokine Growth Factor Rev. 9:259.

Long Name

Family with Sequence Similarity 19, Member A5

Alternate Names

FAM19A5

Entrez Gene IDs

25817 (Human); 106014 (Mouse); 500915 (Rat)

Gene Symbol

TAFA5

UniProt

NP_056196

Additional TAFA5/FAM19A5 Products

Product Documents for Human/Mouse/Rat TAFA5/FAM19A5 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat TAFA5/FAM19A5 Antibody

For research use only

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