Human/Mouse/Rat TOR Antibody

Name: Human/Mouse/Rat TOR Antibody
Brand: R&D Systems
SKU: AB1537

Catalog #: AB1537
1 Citation Datasheet / COA / SDS

Discontinued Product

AB1537 has been discontinued.

View all TOR products.

Detection of Human/Mouse/Rat TOR by Western Blot.

2 Images

All TOR Antibodies

Product Details

Citations (1)

FAQs

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat TOR Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat TOR in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant GST-rat TOR
aa 1223-1290

Formulation

Supplied as a 0.2 μm filtered solution in PBS and Glycerol.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human/Mouse/Rat TOR by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat TOR Polyclonal Antibody (Catalog # AB1537) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TOR at approximately 270 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Simple Western

View Larger

Detection of Human TOR by Simple Western^TM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TOR at approximately 226 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat TOR Polyclonal Antibody (Catalog # AB1537). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: TOR

The Target of Rapamycin (TOR) is a member of the PI 3-kinase-related kinase (PIKK) family. TOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.

Long Name

Mammalian Target of Rapamycin

Entrez Gene IDs

2475 (Human); 287871 (Rat)

Alternate Names

EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse/Rat TOR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1