Human/Mouse/Rat TOR Antibody

AB1537 has been discontinued. View all TOR products.
(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse and rat TOR in Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    E. coli-derived recombinant GST-rat TOR
    aa 1223-1290
  • Formulation
    Supplied as a 0.2 μm filtered solution in PBS and Glycerol.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human/Mouse/Rat TOR by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat TOR Polyclonal Antibody (Catalog # AB1537) followed by HRP‑conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TOR at approximately 270 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human TOR by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TOR at approximately 226 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat TOR Polyclonal Antibody (Catalog # AB1537). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: TOR
The Target of Rapamycin (TOR) is a member of the PI 3-kinase-related kinase (PIKK) family. TOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.
  • Long Name:
    Mammalian Target of Rapamycin
  • Entrez Gene IDs:
    2475 (Human); 287871 (Rat)
  • Alternate Names:
    EC 2.7.11.1; FK506 binding protein 12-rapamycin associated protein 1; FK506 binding protein 12-rapamycin associated protein 2; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; FLJ44809; FRAP; FRAP1; FRAP1FKBP12-rapamycin complex-associated protein 1; FRAP2; Mammalian target of rapamycin; mechanistic target of rapamycin (serine/threonine kinase); Mechanistic target of rapamycin; MTOR; RAFT1; rapamycin and FKBP12 target 1; rapamycin associated protein FRAP2; Rapamycin target protein 1; RAPT1FKBP-rapamycin associated protein; serine/threonine-protein kinase mTOR; TOR
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Select nutrients in the ovine uterine lumen. VI. Expression of FK506-binding protein 12-rapamycin complex-associated protein 1 (FRAP1) and regulators and effectors of mTORC1 and mTORC2 complexes in ovine uteri and conceptuses.
    Authors: Gao H, Wu G, Spencer TE, Johnson GA, Bazer FW
    Biol. Reprod., 2009;81(1):87-100.
    Species: Ovine
    Sample Type: Whole Tissue
    Application: IHC

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