Human/Mouse/Rat TOR Antibody

Name: Human/Mouse/Rat TOR Antibody
Brand: R&D Systems
SKU: AB1537

R&D Systems | Catalog # AB1537

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Citations (1)

Product Documents

Discontinued Product

AB1537 has been discontinued. View all TOR products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Ovine

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all TOR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant GST-rat TOR
aa 1223-1290

Specificity

Detects human, mouse and rat TOR in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat TOR Antibody

Detection of Human/Mouse/Rat TOR by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line and DU145 human prostate carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Mouse/Rat TOR Polyclonal Antibody (Catalog # AB1537) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for TOR at approximately 270 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Detection of Human TOR by Simple Western^TM.

Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for TOR at approximately 226 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human/Mouse/Rat TOR Polyclonal Antibody (Catalog # AB1537). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Applications for Human/Mouse/Rat TOR Antibody

Application

Recommended Usage

Simple Western

20 µg/mL
Sample: MCF‑7 human breast cancer cell line

Western Blot

2 µg/mL
Sample: MCF-7 human breast cancer cell line and DU145 human prostate carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied as a 0.2 μm filtered solution in PBS and Glycerol.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: TOR

The Target of Rapamycin (TOR) is a member of the PI 3-kinase-related kinase (PIKK) family. TOR is the protein target of rapamycin, an anti-rejection drug used in transplantation and promising anti-cancer agent. TOR plays a crucial role in the control of cell growth and proliferation as a downstream target of the PI 3-kinase/Akt signal transduction pathway.