UCH-L3 (Ubiquitin carboxyterminal hydrolase isozyme 3) is a 26‑28 kDa member of the peptidase C12 family of enzymes. It shows wide expression, being noted in adipocytes, renal duct epithelium, neurons, and striated muscle. UCH‑L3 cleaves both monomeric ubiquitin (Ub) from Ub‑protein conjugates, and a GGLRQ peptide from the C‑terminus of the Ub‑like protein NEDD‑8. Notably, UCH-L3 activity is muted in the presence of Ub interacting dimers. Human UCH-L3 is 230 amino acids (aa) in length. It is phosphorylated on Ser75 and Ser130, and contains two ubiquitin-binding sequences between aa 40‑57 and 178‑186. Four potential splice forms have been reported. Two show a four aa deletion between aa 15‑18, with one also containing a 14 aa substitution for aa 179‑230. Two others contain an alternative start site at Met37, one of which is also accompanied by a seven aa substitution for aa 143‑230. Full-length human UCH-L3 shares 98% aa identity with mouse and rat UCH‑L3.
Human/Mouse/Rat UCH‑L3 Alexa Fluor™ Plus 405‑conjugated Antibody
R&D Systems | Catalog # AF6008AFP405
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Species Reactivity
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Antibody Source
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Applications for Human/Mouse/Rat UCH‑L3 Alexa Fluor™ Plus 405‑conjugated Antibody
Western Blot
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Background: UCH-L3
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UniProt
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Product Documents for Human/Mouse/Rat UCH‑L3 Alexa Fluor™ Plus 405‑conjugated Antibody
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Product Specific Notices for Human/Mouse/Rat UCH‑L3 Alexa Fluor™ Plus 405‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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